ID   ECAAD      standard; DNA; PRO; 1444 BP.
XX
AC   X02340; M10241;
XX
NI   g40880
XX
DT   02-JUL-1986 (Rel. 09, Created)
DT   17-FEB-1997 (Rel. 50, Last updated, Version 5)
XX
DE   E. coli R538-1 plasmid aadA gene
DE   for aminoglycoside 3" adenyltransferase AAD(3")(9)
XX
KW   aminoglycoside 3" adenyltransferase; spectinomycin resistance;
KW   streptomycin resistance; transferase.
XX
OS   Escherichia coli
OC   Eubacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
OC   Escherichia.
XX
OG   Plasmid R538-1
XX
RN   [1]
RP   1-1444
RX   MEDLINE; 85191077.
RA   Hollingshead S.K., Vapnek D.;
RT   "Nucleotide Sequence Analysis of a Gene Encoding a
RT   Streptomycin/Spectinomycin Adenyltransferase";
RL   Plasmid 13:17-30(1985).
XX
RN   [2]
RP   256-258
RA   Hollingshead S.;
RT   ;
RL   Submitted (19-MAR-1986) to the EMBL/GenBank/DDBJ databases.
XX
DR   SWISS-PROT; P04826; S3AD_ECOLI.
XX
CC   Data kindly reviewed (19-MAR-1986) by S. Hollingshead
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1444
FT                   /organism="Escherichia coli"
FT                   /plasmid="Plasmid R538-1"
FT   -35_signal      132..137
FT                   /note="putative"
FT   -35_signal      139..144
FT                   /note="putative"
FT   -10_signal      156..162
FT                   /note="putative"
FT   -10_signal      161..167
FT                   /note="putative"
FT   RBS             208..215
FT                   /note="pot. rRNA binding site"
FT   CDS             223..1194
FT                   /db_xref="PID:g581035"
FT                   /db_xref="SWISS-PROT:P04826"
FT                   /product="put. AAD (3)(9) precursor"
FT                   /translation="MVTAQWRFSWLLVMTCFFGVQSMPRASKQQARYAVGRCLMLWSSN
FT                   DVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
FT                   HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPA
FT                   KRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDL
FT                   FEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQ
FT                   PVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
FT   sig_peptide     223..297
FT   mat_peptide     298..1191
FT                   /product="put. AAD"
FT   old_sequence    255..256
FT                   /citation=[1]
FT                   /replace="tcttg"
FT   misc_feature    415..417
FT                   /note="pot. altern. translation initiation (GTG)"
XX
SQ   Sequence 1444 BP; 360 A; 355 C; 392 G; 337 T; 0 other;
     GGCCTGACGA TGCGTGGAGA CCGAAACCTT GCGCTCGTTC GCCACGCCAG GACAGAAATG        60
     CCTCGACTTC GCTGCTGCCC AAGGTTGCCG GGTGACGCAC ACCGTGGAAA CGGATGAAGG       120
     CACGAACCCA GTGGACATAA GCCTCGTTCG GTTCGTAAGC TGTAATGCAA GTAGCGTAAC       180
     TGCCGTCACG CAACTGGTCC AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG       240
     CGGTTTTCAT GGCTTCTTGT TATGACATGT TTTTTTGGGG TACAGTCTAT GCCTCGGGCA       300
     TCCAAGCAGC AAGCGCGTTA CGCCGTGGGT CGATGTTTGA TGTTATGGAG CAGCAACGAT       360
     GTTACGCAGC AGGGCAGTCG CCCTAAAACA AAGTTAAACA TCATGAGGGA AGCGGTGATC       420
     GCCGAAGTAT CGACTCAACT ATCAGAGGTA GTTGGCGTCA TCGAGCGCCA TCTCGAACCG       480
     ACGTTGCTGG CCGTACATTT GTACGGCTCC GCAGTGGATG GCGGCCTGAA GCCACACAGT       540
     GATATTGATT TGCTGGTTAC GGTGACCGTA AGGCTTGATG AAACAACGCG GCGAGCTTTG       600
     ATCAACGACC TTTTGGAAAC TTCGGCTTCC CCTGGAGAGA GCGAGATTCT CCGCGCTGTA       660
     GAAGTCACCA TTGTTGTGCA CGACGACATC ATTCCGTGGC GTTATCCAGC TAAGCGCGAA       720
     CTGCAATTTG GAGAATGGCA GCGCAATGAC ATTCTTGCAG GTATCTTCGA GCCAGCCACG       780
     ATCGACATTG ATCTGGCTAT CTTGCTGACA AAAGCAAGAG AACATAGCGT TGCCTTGGTA       840
     GGTCCAGCGG CGGAGGAACT CTTTGATCCG GTTCCTGAAC AGGATCTATT TGAGGCGCTA       900
     AATGAAACCT TAACGCTATG GAACTCGCCG CCCGACTGGG CTGGCGATGA GCGAAATGTA       960
     GTGCTTACGT TGTCCCGCAT TTGGTACAGC GCAGTAACCG GCAAAATCGC GCCGAAGGAT      1020
     GTCGCTGCCG ACTGGGCAAT GGAGCGCCTG CCGGCCCAGT ATCAGCCCGT CATACTTGAA      1080
     GCTAGACAGG CTTATCTTGG ACAAGAAGAA GATCGCTTGG CCTCGCGCGC AGATCAGTTG      1140
     GAAGAATTTG TCCACTACGT GAAAGGCGAG ATCACCAAGG TAGTCGGCAA ATAATGTCTA      1200
     ACAATTCGTT CAAGCCGACG CCGCTTCGCC GGCGTTAACT CAAGCGATTA GATGCACTAA      1260
     GCACATAATT GCTCACAGCC AAACTATCAG GTCAAGTCTG CTTTTATTAT TTTTAAGCGT      1320
     GCATAATAAG CCCTACACAA ATTGGGAGAT ATATCATGAA AGGCTGGCTT TTTCTTGTTA      1380
     TCGCAATAGT TGGCGAAGTA ATCGCAACAT CCGCATTAAA ATCTAGCGAG GGCTTTCTAA      1440
     GCTT                                                                   1444
//