ID   ISTN5X     standard; DNA; PRO; 1300 BP.
XX
AC   V00618; J01834;
XX
NI   g43748
XX
DT   13-JUL-1983 (Rel. 03, Created)
DT   30-NOV-1997 (Rel. 53, Last updated, Version 18)
XX
DE   Transposon Tn5 fragment encoding neomycin and kanamycin resistance
DE   (neo) and a fragment of the reading frame of a further protein.
XX
KW   drug resistance gene; neomycin resistance; transferase.
XX
OS   Escherichia coli
OC   Eubacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
OC   Escherichia.
XX
RN   [1]
RP   1-1300
RX   MEDLINE; 83106478.
RA   Beck E., Ludwig G., Auerswald E.A., Reiss B., Schaller H.;
RT   "Nucleotide sequence and exact localization of the neomycin
RT   phosphotransferase gene from transposon Tn5";
RL   Gene 19:327-336(1982).
XX
DR   SPTREMBL; Q99399; Q99399.
DR   SWISS-PROT; P00552; KKA2_KLEPN.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1300
FT                   /organism="Escherichia coli"
FT   CDS             151..945
FT                   /db_xref="PID:g43749"
FT                   /db_xref="SWISS-PROT:P00552"
FT                   /product="neomycin phosphotransferase"
FT                   /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
FT                   VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
FT                   SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
FT                   GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
FT                   LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
FT   CDS             942..>1300
FT                   /db_xref="PID:e1192280"
FT                   /note="unidentified reading frame"
FT                   /translation="MSGTLGFEMTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMI
FT                   LQRGDLMLEFFAHPGLDPLASWFSCCLRLDDLAEFYRQCKSVGIQETSSGYPRIHAPEL
FT                   QEWGGTMAALVDPDGT"
XX
SQ   Sequence 1300 BP; 245 A; 374 C; 408 G; 273 T; 0 other;
     ACAGCAAGCG AACCGGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC        60
     AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGATCT       120
     GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT       180
     TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC       240
     TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG       300
     ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG       360
     GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC       420
     TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC       480
     GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC       540
     TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC       600
     GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG       660
     TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT       720
     GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC       780
     CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA       840
     GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT       900
     TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT       960
     TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG      1020
     CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC      1080
     AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCGG GCTCGATCCC CTCGCGAGTT      1140
     GGTTCAGCTG CTGCCTGAGG CTGGACGACC TCGCGGAGTT CTACCGGCAG TGCAAATCCG      1200
     TCGGCATCCA GGAAACCAGC AGCGGCTATC CGCGCATCCA TGCCCCCGAA CTGCAGGAGT      1260
     GGGGAGGCAC GATGGCCGCT TTGGTCGACC CGGACGGGAC                            1300
//