ID   SFKANR     standard; DNA; PRO; 1489 BP.
XX
AC   V01547;
XX
NI   g47033
XX
DT   06-DEC-1983 (Rel. 04, Created)
DT   30-DEC-1994 (Rel. 42, Last updated, Version 3)
XX
DE   Streptococcus faecalis kanamycin resistance
DE   gene encoding a 3'5''-aminoglycoside phosphotransferase of
DE   type III. The gene resides on plasmid pJH1.
XX
KW   drug resistance gene; kanamycin resistance; plasmid; transferase.
XX
OS   Enterococcus faecalis
OC   Eubacteria; Firmicutes; Low G+C gram-positive bacteria; Enterococcus.
XX
RN   [1]
RP   1-1489
RX   MEDLINE; 84029883.
RA   Trieu-Cuot P., Courvalin P.;
RT   "Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding
RT   the 3'5''-aminoglycoside phosphotransferase type III";
RL   Gene 23:331-341(1983).
XX
DR   SWISS-PROT; P00554; KKA3_ENTFA.
XX
CC   Data kindly reviewed (20-OCT-1983) by P. Courvalin
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1489
FT                   /organism="Enterococcus faecalis"
FT   CDS             535..1329
FT                   /db_xref="PID:g47034"
FT                   /db_xref="SWISS-PROT:P00554"
FT                   /note="kanamycin resistance gene"
FT                   /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
FT                   TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
FT                   EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
FT                   PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FT                   FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
XX
SQ   Sequence 1489 BP; 479 A; 252 C; 357 G; 401 T; 0 other;
     GATAAACCCA GCGAACCATT TGAGGTGATA GGTAAGATTA TACCGAGGTA TGAAAACGAG        60
     AATTGGACCT TTACAGAATT ACTCTATGAA GCGCCATATT TAAAAAGCTA CCAAGACGAA       120
     GAGGATGAAG AGGATGAGGA GGCAGATTGC CTTGAATATA TTGACAATAC TGATAAGATA       180
     ATATATCTTT TATATAGAAG ATATCGCCGT ATGTAAGGAT TTCAGGGGGC AAGGCATAGG       240
     CAGCGCGCTT ATCAATATAT CTATAGAATG GGCAAAGCAT AAAAACTTGC ATGGACTAAT       300
     GCTTGAAACC CAGGACAATA ACCTTATAGC TTGTAAATTC TATCATAATT GTGGTTTCAA       360
     AATCGGCTCC GTCGATACTA TGTTATACGC CAACTTTCAA AACAACTTTG AAAAAGCTGT       420
     TTTCTGGTAT TTAAGGTTTT AGAATGCAAG GAACAGTGAA TTGGAGTTCG TCTTGTTATA       480
     ATTAGCTTCT TGGGGTATCT TTAAATACTG TAGAAAAGAG GAAGGAAATA ATAAATGGCT       540
     AAAATGAGAA TATCACCGGA ATTGAAAAAA CTGATCGAAA AATACCGCTG CGTAAAAGAT       600
     ACGGAAGGAA TGTCTCCTGC TAAGGTATAT AAGCTGGTGG GAGAAAATGA AAACCTATAT       660
     TTAAAAATGA CGGACAGCCG GTATAAAGGG ACCACCTATG ATGTGGAACG GGAAAAGGAC       720
     ATGATGCTAT GGCTGGAAGG AAAGCTGCCT GTTCCAAAGG TCCTGCACTT TGAACGGCAT       780
     GATGGCTGGA GCAATCTGCT CATGAGTGAG GCCGATGGCG TCCTTTGCTC GGAAGAGTAT       840
     GAAGATGAAC AAAGCCCTGA AAAGATTATC GAGCTGTATG CGGAGTGCAT CAGGCTCTTT       900
     CACTCCATCG ACATATCGGA TTGTCCCTAT ACGAATAGCT TAGACAGCCG CTTAGCCGAA       960
     TTGGATTACT TACTGAATAA CGATCTGGCC GATGTGGATT GCGAAAACTG GGAAGAAGAC      1020
     ACTCCATTTA AAGATCCGCG CGAGCTGTAT GATTTTTTAA AGACGGAAAA GCCCGAAGAG      1080
     GAACTTGTCT TTTCCCACGG CGACCTGGGA GACAGCAACA TCTTTGTGAA AGATGGCAAA      1140
     GTAAGTGGCT TTATTGATCT TGGGAGAAGC GGCAGGGCGG ACAAGTGGTA TGACATTGCC      1200
     TTCTGCGTCC GGTCGATCAG GGAGGATATC GGGGAAGAAC AGTATGTCGA GCTATTTTTT      1260
     GACTTACTGG GGATCAAGCC TGATTGGGAG AAAATAAAAT ATTATATTTT ACTGGATGAA      1320
     TTGTTTTAGT ACCTAGATTT AGATGTCTAA AAAGCTTTAA CTACAAGCTT TTTAGACATC      1380
     TAATCTTTTC TGAAGTACAT CCGCAACTGT CCATACTCTG ATGTTTTATA TCTTTTCTAA      1440
     AAGTTCGCTA GATAGGGGTC CCGAGCGCCT ACGAGGAATT TGTATCGAT                  1489
//