Class 30 (Agrobacterium tumefaciens tetA(30) gene)
ID AF090987 standard; DNA; PRO; 2767 BP.
XX
AC AF090987;
XX
NI g3860030
XX
DT 12-NOV-1998 (Rel. 57, Created)
DT 12-NOV-1998 (Rel. 57, Last updated, Version 1)
XX
DE Agrobacterium tumefaciens transcriptional repressor TetR (tetR) and
DE drug efflux protein TetA (tetA) genes, complete cds.
XX
KW .
XX
OS Agrobacterium tumefaciens
OC Bacteria; Proteobacteria; alpha subdivision; Rhizobiaceae group;
OC Rhizobiaceae; Agrobacterium.
XX
RN [1]
RP 1-2767
RA Luo Z.-Q., Farrand S.K.;
RT "Cloning and Characterization of a Tetracycline Resistance Determinant
RT Present in Agrobacterium tumefaciens Strain C58";
RL Unpublished.
XX
RN [2]
RP 1-2767
RA Luo Z.-Q., Farrand S.K.;
RT ;
RL Submitted (07-SEP-1998) to the EMBL/GenBank/DDBJ databases.
RL Crop Sciences, University of Illinois at Urbana-Champaign, 1201 West
RL Gregory Drive, Urbana, IL 61801, USA
XX
FH Key Location/Qualifiers
FH
FT source 1..2767
FT /organism="Agrobacterium tumefaciens"
FT /strain="C58"
FT CDS complement(394..1041)
FT /codon_start=1
FT /db_xref="PID:g3860031"
FT /note="DNA binding protein"
FT /transl_table=11
FT /gene="tetR"
FT /product="transcriptional repressor TetR"
FT /translation="MAIGRDRIVDEALRLLNEVGIDKLSTRKLAERLGVQQPALYWHFR
FT NKAELLDAINSEMLLRYHCDRLPKPGQDWITFTLANARSIRKTLLTVRDGARLTAGTRP
FT SVAEFADVESVLQLYVETGFSAEEAFGIAICITRYVVGYVLEEQGERERDRMDQHSPNT
FT DIMAELAQFPLLAKALESFPTVGTVNTEAVFESGLSYLLAGMKEKLREKQSR"
FT RBS complement(1051..1056)
FT /note="putative"
FT /gene="tetR"
FT -35_signal 1067..1072
FT /note="putative"
FT /gene="tetA"
FT -10_signal complement(1078..1083)
FT /note="putative"
FT /gene="tetR"
FT -10_signal 1090..1095
FT /note="putative"
FT /gene="tetA"
FT protein_bind 1094..1108
FT /note="putative operator"
FT /gene="tetA"
FT /bound_moiety="TetR"
FT -35_signal complement(1102..1107)
FT /note="putative"
FT /gene="tetR"
FT RBS 1117..1122
FT /note="putative"
FT /gene="tetA"
FT CDS 1130..2314
FT /codon_start=1
FT /db_xref="PID:g3860032"
FT /transl_table=11
FT /gene="tetA"
FT /function="drug resistance pump"
FT /product="drug efflux protein TetA"
FT /translation="MNKALIVILSTVALDAIGAGLIFPILPDILVEVTGGGDIGFLYGV
FT MLGVFAVMQFVFSPILGALSDRFGRRPVLLLSLAGTLLDYLVMAFSPLGWVLVVGRAMA
FT GITSANMAVASAYITDITPAEQRAQRFGTVGAVMSLGFIIGPVIGGVIGAWWLRAPFLV
FT AALFNGLNLFVALFVLPESRKAGPGKFAFKELNPLAPLVWLWNFKPLLPLVTVSVVFGL
FT VAAIPGTIWVLYGAERFGWDSVHMGLSLSVFGVSGALAQAFLVGPLSRRFGDLGTLMIG
FT VGFDMLAYMLMAFANQSWMGYAVAPLFALGGVAMPALQSLVTSRVSDDQQGQLQGVLAS
FT LMSLAGIIGPVLTTAVFFSTKSIWIGTIWLVGAALYLLALPLFATVKTPKAVAA"
XX
SQ Sequence 2767 BP; 532 A; 777 C; 790 G; 668 T; 0 other;
GAATTTGCTC AAGAAGACTT GGAGTAGTCA TCCGCTGCTT TCAACATCCA TTGCGGTCTT 60
CTCACTCGGA CGCAACTACG GCAGCTTCGG GCTCGGAAAC GGTCATGGAG AACGGCCCCG 120
CTCATGGCAG CTATTTTCAT CCTTAGCCTC CAAGACTGGA CCATCCGTTC ACGGTCCCAT 180
CCAGGCCATT CCATAGAGTT ATACTTTCGA AAAGCGCAAC ATTCATTTTT ACGTCAACAA 240
ATGCGGATTC AAAATTGTCG AATATTATAA TTCGCGGCAT CCTGATCCGC ACGACACAGA 300
AGAGAGTGAT GCCAGCGACA GTGGCATGTT AAGGTTTGAA AAAGACATGT TGTATACCGG 360
AAACTGATTG CACGTCCGCG CCAGAGTCTT CGCCTATCTA GACTGTTTCT CCCGCAGCTT 420
CTCCTTCATA CCGGCCAGCA GGTAACTTAG CCCACTCTCG AACACGGCTT CGGTGTTGAC 480
CGTTCCAACC GTGGGGAAGC TTTCAAGAGC TTTTGCAAGC AGGGGGAACT GTGCGAGTTC 540
TGCCATGATG TCGGTATTGG GCGAGTGTTG ATCCATCCGA TCCCGTTCGC GCTCTCCCTG 600
TTCTTCGAGC ACATAGCCGA CCACATAGCG CGTGATGCAA ATGGCGATCC CAAATGCCTC 660
CTCTGCGGAA AATCCCGTCT CGACATAGAG CTGCAAGACG CTTTCCACAT CGGCAAACTC 720
GGCGACGGAA GGCCGTGTAC CTGCGGTTAG CCGCGCGCCA TCGCGCACGG TGAGCAAGGT 780
CTTGCGAATG CTGCGGGCAT TGGCCAGCGT GAACGTAATC CAATCCTGGC CGGGTTTGGG 840
CAGGCGATCG CAGTGATAAC GCAGCAGCAT TTCGGAATTG ATGGCGTCGA GCAATTCCGC 900
CTTGTTGCGG AAGTGCCAGT AGAGCGCCGG CTGCTGGACG CCCAGCCGTT CCGCGAGCTT 960
GCGGGTGGAC AGCTTGTCTA TTCCCACCTC GTTCAGGAGC CGCAGGGCCT CATCGACAAT 1020
GCGATCACGT CCGATAGCCA TGGATAGAAT CCCTCTTGAC TTATCATTGA TAAGGGAGCT 1080
TATCGCCGAT AAATTTATCA CTGATAAAGC TTTATAGGGA TGCCATACCA TGAACAAGGC 1140
CCTTATCGTT ATTCTCTCAA CCGTTGCCCT CGACGCCATT GGCGCAGGCC TGATCTTCCC 1200
GATCCTGCCG GACATCTTGG TCGAGGTGAC TGGCGGCGGC GACATCGGGT TCCTCTATGG 1260
GGTCATGCTG GGGGTATTCG CCGTCATGCA ATTTGTGTTC TCGCCGATCC TTGGTGCGCT 1320
CAGCGACCGG TTCGGTCGGC GCCCGGTCTT GTTGCTTTCT TTGGCCGGTA CCCTGCTTGA 1380
TTACCTTGTT ATGGCATTTT CCCCGCTCGG CTGGGTGCTC GTCGTCGGGC GGGCCATGGC 1440
GGGGATCACC AGCGCAAATA TGGCGGTGGC AAGCGCCTAC ATCACTGACA TCACCCCAGC 1500
CGAGCAGCGC GCGCAGCGGT TTGGCACGGT TGGTGCCGTG ATGAGCCTGG GCTTTATCAT 1560
CGGTCCCGTC ATTGGTGGCG TCATTGGCGC CTGGTGGCTT CGGGCACCAT TTCTTGTGGC 1620
AGCCCTGTTC AATGGCCTCA ACCTGTTCGT CGCGCTGTTT GTTCTGCCGG AAAGCCGAAA 1680
GGCCGGTCCG GGCAAGTTTG CGTTCAAGGA ACTTAACCCG TTGGCGCCAT TGGTGTGGCT 1740
TTGGAATTTC AAGCCGCTCC TGCCACTTGT AACCGTCTCT GTCGTCTTCG GTCTGGTGGC 1800
CGCCATCCCG GGAACGATCT GGGTGCTCTA TGGCGCCGAG CGGTTCGGAT GGGATTCGGT 1860
GCATATGGGC CTGTCGCTAT CGGTTTTCGG CGTCAGTGGC GCCCTGGCGC AGGCCTTTCT 1920
CGTCGGGCCG CTCTCGCGCC GCTTTGGTGA TTTGGGCACG TTGATGATCG GCGTTGGCTT 1980
TGACATGCTG GCTTATATGC TGATGGCCTT CGCCAACCAG AGCTGGATGG GCTACGCGGT 2040
AGCGCCCCTG TTTGCATTGG GCGGCGTTGC CATGCCGGCG CTGCAATCTC TGGTAACCAG 2100
CCGCGTGAGC GATGATCAGC AGGGCCAGTT GCAGGGCGTG CTCGCCAGCC TCATGAGCCT 2160
GGCGGGTATA ATAGGGCCGG TGCTGACCAC CGCAGTGTTC TTTTCCACCA AAAGCATCTG 2220
GATCGGGACG ATCTGGCTGG TGGGTGCCGC ACTTTATCTT CTCGCCTTGC CGCTGTTCGC 2280
AACGGTGAAA ACCCCGAAGG CTGTGGCGGC TTAAAGACTC CGGACGTCAT GCGCCTCGTG 2340
TTTATCTTCG CCGCCGATTT GATGTCATCG CTTCGAATCA AACAGGTACG AGCGTTGAAC 2400
TGGAAATCAG AACGCCAGTT CAACGGCATC TCCAAGCGAC GCTTTTCGGC CGATCACGAT 2460
TGCGAGCGCA TATTAGCTAT GGCTGGAAGG TCATGAAGGA TCATGGCTGC CGCGCCGAAA 2520
GCCACGGTAT CCGCAGCAGA GACTTCGGGC AAAAGAATTG CCGGCTGAAG AACTTTCGCA 2580
CCCTCGAACG GATCGGAAAC TGAAAAATCG AGCCAGGGGC ATAATTGTTC CCGGATCATG 2640
CGGGGCAGGG AGCCCCCGAC ATATATTGCC TCAGGGGCCA GAACAGCGCT GAGGGAAAAT 2700
CCCAGTCTGG CAGTTTCTGG CCCGGCCCGA TTTATCCACG TTATCTACAA GCTCTTGATT 2760
GCTGTTC 2767
//