Class H (Pasteurella multocida - plasmid pPMT1 -tetA and tetR genes)
ID PMPPMT1 standard; DNA; PRO; 4828 BP.
XX
AC Y15510;
XX
NI e1352188
XX
DT 16-NOV-1998 (Rel. 57, Created)
DT 16-NOV-1998 (Rel. 57, Last updated, Version 1)
XX
DE Pasteurella multocida pPMT1 plasmid DNA for tnp, tetR, tetH, tnp
DE genes
XX
KW insertion element; inverted repeat; repeat region; tetH gene; tetR gene;
KW tetracycline resistance efflux; tetracycline resistance repressor;
KW tnp gene; transposase; transposon.
XX
OS Pasteurella multocida
OC Bacteria; Proteobacteria; gamma subdivision; Pasteurellaceae;
OC Pasteurella.
XX
OG Plasmid pPMT1
XX
RN [1]
RA Kehrenberg C., Werckenthin C., Schwarz S.;
RT "Tn5706, a transposon-like element from Pasteurella multocida mediating
RT tetracycline resistance";
RL Antimicrob. Agents Chemother. 42:2116-2118(1998).
XX
RN [2]
RP 1-4828
RA Schwarz S.P.;
RT ;
RL Submitted (13-NOV-1997) to the EMBL/GenBank/DDBJ databases.
RL S.P. Schwarz, Institut fuer Kleintierforschung Celle/Merbitz (FAL),
RL Doernbergstr. 25-27, 29223 Celle, FRG
XX
CC Related sequence U00792
XX
FH Key Location/Qualifiers
FH
FT source 1..4828
FT /organism="Pasteurella multocida"
FT /isolate="151"
FT /plasmid="pPMT1"
FT source 226..4603
FT /organism="Pasteurella multocida"
FT /note="entire transposon"
FT source 226..1288
FT /organism="Pasteurella multocida"
FT /note="insertion element 1"
FT repeat_region 226..243
FT /note="repeat 1'"
FT /rpt_type=INVERTED
FT CDS complement(269..955)
FT /db_xref="PID:e1352189"
FT /gene="tnp"
FT /product="transposase"
FT /translation="MLGMMKKDFPDLPSYSRFIELVPRALVPLCAYLKSLMGSCTGISF
FT VDATKLSVCHNRRIKRHKVFEGIAQRGKTSMGWFYGFKLHAIINHHGELLSIRVTPGNI
FT DDREPLRQGLANDIFGKLFGDRGYVSQDLKDKLFNDFNIDFITKLRRKMKQQILKPIDE
FT ALLNGRSLIETVFDELKNLCQIEHSRHRSFTGFAANLLAGLIAYCWFPFKPTLKNVSAY
FT GQAAKN"
FT repeat_region 1271..1288
FT /note="repeat 1''"
FT /rpt_type=INVERTED
FT CDS complement(1384..2007)
FT /db_xref="PID:e1352190"
FT /gene="tetR"
FT /product="tetracycline resistance repressor protein"
FT /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWHV
FT KNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAGTR
FT PSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREKVET
FT DTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
FT CDS 2099..3301
FT /db_xref="PID:e1352191"
FT /gene="tetH"
FT /product="tetracycline resistance efflux protein of class
FT H"
FT /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYGV
FT LLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRIIA
FT GITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMPFIF
FT AAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATYFIIQ
FT LIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLHIFFQAIVAGKLAQKWGEKTTIMI
FT SMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGKLQGTLV
FT SLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTTPKAVIST
FT P"
FT source 3541..4603
FT /organism="Pasteurella multocida"
FT /note="insertion element 2"
FT repeat_region 3541..3558
FT /note="repeat 2'"
FT /rpt_type=INVERTED
FT CDS 3874..4560
FT /db_xref="PID:e1352192"
FT /gene="tnp"
FT /product="transposase"
FT /translation="MLGMMKKDFPDLPSYSRFIELVPRALVPLCAYLKSLMGSCTGISF
FT VDATKLSVCHNRRIKRHKVFEGIAQRGKTSMGWFYGFKLHAIINHHGELLSIRVTPGNI
FT DDREPLRQGLANDIFGKLFGDRGYVSQDLKDKLFNDFNIDFITKLRRNMKQQILKPIDE
FT ALLNGRSLIETVFDELKNLCQIEHSRHRSFTGFAANLLAGLIAYCWFPFKPTLKNVSAY
FT GQAAKN"
FT repeat_region 4586..4603
FT /note="repeat 2''"
FT /rpt_type=INVERTED
XX
SQ Sequence 4828 BP; 1423 A; 957 C; 927 G; 1521 T; 0 other;
AGGGAGCGTA GCGACTGCGG TAGGTATCGT GATGGTTGAA TTACAAATTA TTCTCTACAA 60
TGTGATTATT ATCGGCTTTT TGATTGGCCG GCATTTATGT TTATTTGAAA TGGCAACGTA 120
AAAAGCGTAT CGAAAACATA CTTGATCGGA TGGAAGCCAG TTATCAGAAA TACGCCCCAC 180
CGAATGCAGA GCTGCGATTA AGACAAAAAC AAGCTGAGTA TGATAACCTG AGTTCGGGAT 240
AAGAGCTTGC CTAAATCATT GAAACAGCTT AGTTTTTTGC AGCTTGTCCA TAAGCCGACA 300
CGTTTTTGAG TGTCGGCTTA AAGGGAAACC AACAGTAAGC AATGAGTCCT GCTAAAAGAT 360
TAGCAGCGAA CCCTGTGAAG CTTCGATGCC TTGAGTGTTC AATCTGACAT AGGTTTTTAA 420
GCTCATCAAA AACAGTTTCA ATTAACGAGC GTCCGTTAAG TAACGCCTCA TCAATAGGTT 480
TAAGAATCTG CTGTTTCATT TTCCTTCGAA GCTTGGTTAT AAAATCGATA TTGAAGTCAT 540
TAAACAGTTT ATCTTTTAAA TCTTGACTGA CATACCCTCG ATCACCAAAC AGTTTACCGA 600
ATATATCATT TGCTAGGCCC TGTCTTAGCG GCTCTCTATC ATCTATATTT CCCGGAGTGA 660
CTCTAATCGA TAATAACTCA CCGTGATGAT TGATAATCGC GTGCAGTTTA AATCCGTAAA 720
ACCAACCCAT ACTTGTCTTA CCTCTTTGAG CAATCCCCTC AAAGACCTTA TGACGCTTAA 780
TCCGCCGGTT ATGGCATACA GATAGTTTGG TGGCATCCAC AAAGCTAATA CCTGTGCAAC 840
TTCCCATCAA GCTTTTAAGA TAAGCACACA AAGGCACAAG AGCACGGGGT ACTAATTCAA 900
TAAAACGTGA GTAGCTTGGT AAGTCCGGAA AATCTTTTTT CATCATGCCT AGCATATGAT 960
GATAGTAAAA CCCTTTAAAT TGACGGTAAC GTAATTGATG AAACAGCTTT AAATTGACGG 1020
TAACGTAATT GATGAAACAG TACCAATATA GTCATTATCT CTGGTACACT TATCTTGCAT 1080
GCTCTTAATC GCTTTGTCCC ATTTTCGATC AGCTGACGTT CAAATTGTGG TTTGAATGCT 1140
TGGTAAAAGT CGTCAATGTG GCAGTATAAT TCGGTTAGGT TGTCCATGTA AGAAGTCCTT 1200
TTTGTTGAGT TTTTATTAGT ACATTAACTT TAACAATTTT GGACTTCTTT TTTTTGCCTT 1260
TTTTGACCTG CTTATCCCGA ACTCAGGTTA TTTACAATTT AATGCAGGTT GTATTGCTCA 1320
CATTGGTATC GCTGACATAC AAGGGCAACT AATTAGCCAA AAAGGGCGTC AGCGAATTTA 1380
GATTTATTTA GCGCTCTTTA ATACTGTTTC AAGTCCAGAG ATCATCACAT CAAGGACAAA 1440
CAAAAATGCA GCATCACCAT TATCACTATC CATAATTGCA ACGGCTTGGG TTAATAATGG 1500
CGGATAGGCA ACAGTATCCG TCTCTACTTT TTCACGCTCT TTTTGGCTTT CTTGATGCTC 1560
TTGAGTTTCC AGTACGGAGC CTAATGTAAA ATGCGCAATA GAGCTTAATG CATACACGGC 1620
TTGAGATAGA CTAAACCCAG CATCACACAA AAACTGTAGT TGCTGTTCTG ATGTCTCAAA 1680
TTGACTTTCA GAGGGGCGTG TTCCCGCATG AATTTTGCCA CCATCACGAT ACATTAATAA 1740
GGCTTGGCGG AAGCTTTTCG CGTTATTTCG CAAAAAGTCC TGCCATGTTT CATTCGGCAA 1800
TGGCAAAACA TGATGATGGT GCTTTTGCAA AATAGTTTCT GCTAATGCAT CTAACAAAGC 1860
GCGTTTATTT TTTACATGCC AATACAATGT GGGTTGTTCC ACACCTATTT TTTGCGCCAG 1920
CTTACGCGTT GTTAATCCTT CAATACCAAC TTCATTAAGT AAAATCAACG CATTATCAAT 1980
AACTTGTTCT TTATCTAGCT TTGCCATTAC CTACCTCAAA ATAAAATAGC CTTGACAATC 2040
TATCACTGAT AGAGATATTT TACACCCACT CTATCACTGA TAGATTTTTA GGATCTCAAT 2100
GAATAAATCA ATTATTATTA TACTGCTGAT CACCGTATTA GATGCCATTG GTATCGGGCT 2160
TATCATGCCA GTACTCCCTA CTCTATTAAA TGAATTTGTC AGTGAAAATT CACTGGCAAC 2220
CCATTACGGT GTGCTATTAG CGCTCTATGC TACCATGCAG GTTATTTTTG CTCCTATTCT 2280
AGGACGACTG TCTGATAAAT ACGGCAGAAA ACCCATCTTG CTGTTTTCCC TTTTAGGCGC 2340
GGCACTCGAC TATCTTTTAA TGGCATTCTC AACCACACTT TGGATGCTCT ATATTGGGCG 2400
CATCATTGCG GGGATCACAG GCGCAACAGG TGCCGTATGT GCATCAGCGA TGAGTGATGT 2460
GACTCCCGCT AAAAATCGAA CTCGCTATTT TGGTTTCTTA GGTGGTGCTT TTGGTGTTGG 2520
CCTTATTATC GGCCCAATGC TAGGGGGATT ATTAGGTGAT ATCAGTGCTC ATATGCCATT 2580
TATTTTTGCC GCTATTTCAC ACTCGATATT ATTAATACTC TCTTTGCTCT TTTTCCGAGA 2640
AACACAAAAA AGAGAAGCGC TTGTTGCCAA TAGGACACCT GAAAACCAAA CTGCCTCAAA 2700
TACAGTCACT GTTTTTTTTA AGAAAAGCCT CTACTTTTGG TTAGCAACCT ATTTTATTAT 2760
CCAGCTTATC GGGCAAATTC CTGCCACCAT CTGGGTGCTG TTTACACAAT ATCGTTTTGA 2820
TTGGAACACA ACTTCTATCG GTATGTCTTT GGCGGTTCTG GGTGTATTAC ATATTTTCTT 2880
TCAGGCGATT GTCGCTGGGA AATTGGCACA AAAATGGGGC GAAAAAACCA CCATTATGAT 2940
CAGTATGTCT ATTGATATGA TGGGCTGTTT ATTATTAGCG TGGATAGGCC ACGTTTGGGT 3000
CATCTTACCA GCATTAATTT GCTTAGCGGC AGGAGGTATG GGGCAACCCG CATTACAAGG 3060
TTATTTATCA AAATCTGTCG ATGATAATGC GCAAGGGAAA TTACAAGGTA CTCTGGTGAG 3120
CCTAACCAAT ATTACCGGGA TCATTGGTCC CCTTTTATTT GCCTTTATTT ATAGTTATAG 3180
CGTCGCTTAT TGGGATGGTC TGTTATGGCT GATGGGGGCA ATACTTTATG CTATGTTGCT 3240
TATTACCGCT TATTTTCACC AAAGAAAAAC CACACCTAAA GCTGTTATTT CAACCCCTTA 3300
ATACTAGAAA TTATAAAGAT CATAAAAGTC CTGTCCGCTC TTAATAACGT TGATAGGACT 3360
TTATTTTTAT CGCAATTAAT AAGCCTCTTT TACCCAAGGC TTACTGGCTG CAATCATAAA 3420
CATCGGCGTT GAACGATAAA GCAGGTAAGG GAGTCGTTTA GTTACATAGT TATTTTATGA 3480
TAGTTATTTT CAATCATATT CACCCTGAGT AAAAAAAACA AATATTTCAT TATTAATTTA 3540
ACCTGAGTTC GGGATAAGCA GGTCAAAAAA GGCAAAAAAA AGAAGTCCAA AATTGTTAAA 3600
GTTAATGTAC TAATAAAAAC TCAACAAAAA GGACTTCTTA CATGGACAAC CTAACCGAAT 3660
TATACTGCCA CATTGACGAC TTTTACCAAG CATTCAAACC ACAATTTGAA CGTCAGCTGA 3720
TCGAAAATGG GACAAAGCGA TTAAGAGCAT GCAAGATAAG TGTACCAGAG ATAATGACTA 3780
TATTGGTACT GTTTCATCAA TTACGTTACC GTCAATTTAA AGCTGTTTCA TCAATTACGT 3840
TACCGTCAAT TTAAAGGGTT TTACTATCAT CATATGCTAG GCATGATGAA AAAAGATTTT 3900
CCGGACTTAC CAAGCTACTC ACGTTTTATT GAATTAGTAC CCCGTGCTCT TGTGCCTTTG 3960
TGTGCTTATC TTAAAAGCTT GATGGGAAGT TGCACAGGTA TTAGCTTTGT GGATGCCACC 4020
AAACTATCTG TATGCCATAA CCGGCGGATT AAGCGTCATA AGGTCTTTGA GGGGATTGCT 4080
CAAAGAGGTA AGACAAGTAT GGGTTGGTTT TACGGATTTA AACTGCACGC GATTATCAAT 4140
CATCACGGTG AGTTATTATC GATTAGAGTC ACTCCGGGAA ATATAGATGA TAGAGAGCCG 4200
CTAAGACAGG GCCTAGCAAA TGATATATTC GGTAAACTGT TTGGTGATCG AGGGTATGTC 4260
AGTCAAGATT TAAAAGATAA ACTGTTTAAT GACTTCAATA TCGATTTTAT AACCAAGCTT 4320
CGAAGGAATA TGAAACAGCA GATTCTTAAA CCTATTGATG AGGCGTTACT TAACGGACGC 4380
TCGTTAATTG AAACTGTTTT TGATGAGCTT AAAAACCTAT GTCAGATTGA ACACTCAAGG 4440
CATCGAAGCT TCACAGGGTT CGCTGCTAAT CTTTTAGCAG GACTCATTGC TTACTGTTGG 4500
TTTCCCTTTA AGCCGACACT CAAAAACGTG TCGGCTTATG GACAAGCTGC AAAAAACTAA 4560
GCTGTTTCAA TGATTTAGGC AAGCTCTTAT CCCGAACTCA GGTTATGATA AAAAGCACCC 4620
GTTCAAAGCT CTCAAACGAA AGTTTATGAA CAAAATAAAA TGGCTGCTTT AAATCAGTAG 4680
ATTAAGAATC ATTCAAATCC ATATGTTCCA CACCCTGTCT GGGCAGGGTG TAGAACATAT 4740
ATTCTCGTTC AAGCCTTATG TATCAAGAGC CAGTCTAGCC CCTTTTTTCG ACAGGGTGCG 4800
CACCATACAA ATTTAAGTCT ATTTTTTG 4828
//