Class L (Bacillus subtilis GSY908 chromosomal tet(L) gene)

ID   BSTET      standard; DNA; PRO; 1579 BP.
XX
AC   X08034;
XX
NI   g40207
XX
DT   08-AUG-1988 (Rel. 17, Created)
DT   15-DEC-1995 (Rel. 46, Last updated, Version 9)
XX
DE   Bacillus subtilis GSY908 tet gene for tetracycline-resistance
XX
KW   antibiotic resistance; tet gene; tetracycline resistance.
XX
OS   Bacillus subtilis
OC   Eubacteria; Firmicutes; Low G+C gram-positive bacteria; Bacillaceae;
OC   Bacillus.
XX
RN   [1]
RP   1-1579
RA   Shishido K.;
RT   ;
RL   Submitted (09-JUL-1988) to the EMBL/GenBank/DDBJ databases.
RL   Shishido K., Department of Life Sciences, Faculty of Science, Tokyo
RL   Institute of Technology, Nagatsuta, Yokohama 227, Japan.
XX
RN   [2]
RA   Sakaguchi R., Amano H., Shishido K.;
RT   "Nucleotide sequence homology of the tetracycline-resistance determinant
RT   naturally maintained in Bacillus subtilis Marburg 168 chromosome and the
RT   tetracycline-resistance gene of B.subtilis plasmid pNS1981";
RL   Biochim. Biophys. Acta 950:441-444(1988).
XX
DR   SWISS-PROT; P23053; LPTR_BACSU.
DR   SWISS-PROT; P23054; TCRB_BACSU.
XX
CC   
CC   The reported sequence overlaps with that reported by Shishido
CC   et al. in Journal of Protein Chemistry 6:473-478(1988).
CC   There is a 80% homology between chromosomal tet gene (tetBS908)
CC   and plasmid tet gene (tetpNS1981).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1579
FT                   /organism="Bacillus subtilis"
FT                   /strain="GSY908 (Marburg 168)"
FT                   /clone="tetBS908"
FT   promoter        35..40
FT                   /note="put.-35 region"
FT   promoter        58..63
FT                   /note="put.-10 region"
FT   prim_transcript 69..>1564
FT   RBS             78..84
FT   CDS             92..154
FT                   /db_xref="PID:g40208"
FT                   /db_xref="SWISS-PROT:P23053"
FT                   /product="leader peptide"
FT                   /translation="MKCNKMNRVQLKEGSVSMTL"
FT   RBS             176..180
FT   CDS             188..1564
FT                   /db_xref="PID:g580940"
FT                   /db_xref="SWISS-PROT:P23054"
FT                   /product="TET protein"
FT                   /translation="MNTSYSQSTLRHNQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFN
FT                   KLPASANWVNTAFMLTFSIGTALYGKLSDQLGIKNLLLFGIMVNGLGSIIGFVGHSFFP
FT                   ILILARFIQGIGAAAFPALVMVVVARYIPKENRGKAFGLIGSLVAMGEGVGPAIGGMVA
FT                   HYIHWSYLLLIPTATIITVPFLIKLLKKEERIRGHIDMAGIILMSAGIVFFMLFTTSYR
FT                   FSFLIISILAFFIFVQHIRKAQDPFVDPELGKNVFFVIGTLCGGLIFGTVAGFVSMVPY
FT                   MMKDVHHLSTAAIGSGIIFPGTMSVIIFGYIGGLLVDRKGSLYVLTIGSALLSSGFLIA
FT                   AFFIDAAPWIMTIIVIFVFGGLSFTKTVISTVVSSSLKEKEAGAGMSLLNFTSFLSEGT
FT                   GIAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNMLILFAGIIVICWLVILNVYKRSRRHG
FT                   "
XX
SQ   Sequence 1579 BP; 432 A; 273 C; 344 G; 530 T; 0 other;
     GCATCACCCA AATTTTTATA AAAAAAGTAA AAGTTTAATC CTTAGTCTAT ATATAATAAG        60
     ATCATATCAA TCAAATGTAG GGGGAGTTAT TATGAAGTGC AATAAAATGA ACAGGGTACA       120
     ATTAAAAGAG GGAAGCGTAT CAATGACCCT ATAAACTGCG TCTGCCCTCA TTAATGGAGG       180
     GGAAATTGTG AATACGTCTT ATTCACAGTC AACTTTACGG CACAATCAAG TTTTGATTTG       240
     GCTTTGTGTT CTATCATTTT TCAGCGTATT AAATGAAATG GTTCTGAACG TCTCATTACC       300
     TGATATTGCC AACGAGTTTA ATAAACTGCC AGCAAGTGCA AACTGGGTGA ATACAGCCTT       360
     TATGTTAACC TTCTCCATTG GAACAGCTCT ATATGGAAAG CTATCAGACC AGCTGGGCAT       420
     CAAAAATCTG CTTCTATTTG GGATTATGGT AAATGGCTTA GGGTCGATCA TTGGATTTGT       480
     TGGACACTCT TTCTTTCCTA TTCTCATTCT AGCCCGATTT ATTCAAGGAA TTGGTGCAGC       540
     CGCATTCCCA GCTCTTGTGA TGGTTGTCGT TGCGCGCTAT ATTCCAAAAG AGAACAGGGG       600
     GAAAGCATTT GGCCTGATCG GGTCCCTGGT AGCAATGGGA GAAGGTGTTG GGCCAGCTAT       660
     TGGCGGAATG GTTGCTCATT ATATCCATTG GTCGTATTTG CTGCTTATTC CAACTGCAAC       720
     AATTATCACC GTTCCATTCC TTATAAAATT GTTGAAAAAG GAAGAGAGAA TAAGAGGACA       780
     CATTGATATG GCAGGGATTA TATTGATGTC TGCAGGTATC GTATTTTTTA TGCTGTTTAC       840
     AACATCTTAT AGATTTTCTT TTCTGATCAT CAGTATCCTT GCTTTCTTCA TATTTGTGCA       900
     ACACATTAGG AAAGCTCAGG ACCCTTTTGT TGACCCTGAA TTAGGGAAAA ACGTCTTTTT       960
     TGTGATTGGA ACCCTTTGCG GAGGACTAAT ATTTGGTACA GTAGCAGGAT TTGTCTCTAT      1020
     GGTTCCTTAC ATGATGAAAG ATGTTCACCA TTTAAGTACT GCAGCAATTG GAAGCGGCAT      1080
     TATTTTCCCC GGAACAATGA GTGTCATCAT CTTTGGTTAC ATTGGGGGAT TGCTTGTTGA      1140
     TCGGAAAGGT TCCTTGTATG TACTAACTAT TGGAAGTGCA TTGCTTTCTT CCGGCTTTTT      1200
     AATCGCTGCC TTTTTTATAG ATGCAGCACC ATGGATCATG ACAATTATAG TAATTTTTGT      1260
     TTTTGGGGGG CTATCTTTCA CAAAAACAGT TATATCTACA GTTGTGTCTA GCAGTTTGAA      1320
     AGAAAAGGAA GCGGGCGCTG GAATGAGTTT GCTTAATTTC ACGAGCTTTT TATCAGAGGG      1380
     AACGGGTATT GCGATTGTAG GCGGTTTATT ATCTATCGGC TTCCTAGATC ATAGGCTGCT      1440
     GCCTATTGAC GTTGATCACT CTACCTATTT GTATAGCAAT ATGCTCATAC TTTTTGCAGG      1500
     AATCATTGTT ATTTGTTGGC TAGTAATCTT GAATGTATAT AAACGTTCGC GGAGACATGG      1560
     CTAAATGAGT GAAAGGATC                                                   1579
//