Class L (Bacillus cereus - plasmid pBC16 - tet(L) gene)

ID   BCTET      standard; DNA; PRO; 1644 BP.
XX
AC   X51366;
XX
NI   g39459
XX
DT   16-MAY-1990 (Rel. 24, Created)
DT   12-SEP-1993 (Rel. 36, Last updated, Version 2)
XX
DE   Bacillus cereus plasmid pBC16 tetracycline resistance gene
XX
KW   plasmid; tet gene; TET protein; tetracycline resistance.
XX
OS   Bacillus cereus
OC   Eubacteria; Firmicutes; Low G+C gram-positive bacteria;
OC   Bacillaceae; Bacillus.
XX
OG   Plasmid pBC16
XX
RN   [1]
RP   1-1644
RA   Palva A.;
RT   ;
RL   Submitted (05-JAN-1990) to the EMBL/GenBank/DDBJ databases.
RL   Palva A., Genesit Ltd, Valimotie 7, SF-00380 Helsinki, Finland.
XX
RN   [2]
RP   1-1644
RX   MEDLINE; 90221899.
RA   Palva A., Vidgren G., Simonen M., Rintala H., Laamanen P.;
RT   "Nucleotide sequence of the tetracycline resistance gene of
RT   pBC16";
RL   Nucleic Acids Res. 18:1635-1635(1990).
XX
DR   SWISS-PROT; P05658; LPTR_BACST.
DR   SWISS-PROT; P11063; TCR_STRPN.
XX
CC   The gene shows high homology with the tet gene of pTHT15
XX
CC   Data kindly reviewed (31-MAY-1990) by Palva A.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1644
FT                   /organism="Bacillus cereus"
FT                   /plasmid="Plasmid pBC16"
FT   promoter        30..35
FT                   /note="-35 region"
FT   promoter        53..58
FT                   /note="-10 region"
FT   RBS             74..79
FT                   /note="ribosome binding site"
FT   CDS             88..150
FT                   /note="open reading frame (20 AA)"
FT                   /db_xref="PID:g39460"
FT                   /db_xref="SWISS-PROT:P05658"
FT                   /translation="MKCNECNRVQLKEGSVSLTL"
FT   RBS             172..180
FT                   /note="ribosome binding site"
FT   CDS             184..1557
FT                   /note="TET protein, tetracyclin resistance (AA 1-458)"
FT                   /db_xref="PID:g580765"
FT                   /db_xref="SWISS-PROT:P11063"
FT                   /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
FT                   KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
FT                   LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
FT                   HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
FT                   ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
FT                   MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
FT                   SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
FT                   GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
FT                   "
FT   terminator      1566..1610
FT                   /note="transcription terminator"
XX
SQ   Sequence 1644 BP; 476 A; 244 C; 323 G; 601 T; 0 other;
     TATTGTTGTA TAAGTGATGA AATACTGAAT TTAAAACTTA GTTTATATGT GGTAAAATGT        60
     TTTAATCAAG TTTAGGAGGA ATTAATTATG AAGTGTAATG AATGTAACAG GGTTCAATTA       120
     AAAGAGGGAA GCGTATCATT AACCCTATAA ACTACGTCTG CCCTCATTAT TGGAGGGTGA       180
     AATGTGAATA CATCCTATTC ACAATCGAAT TTACGACACA ACCAAATTTT AATTTGGCTT       240
     TGCATTTTAT CTTTTTTTAG CGTATTAAAT GAAATGGTTT TGAACGTCTC ATTACCTGAT       300
     ATTGCAAATG ATTTTAATAA ACCACCTGCG AGTACAAACT GGGTGAACAC AGCCTTTATG       360
     TTAACCTTTT CCATTGGAAC AGCTGTATAT GGAAAGCTAT CTGATCAATT AGGCATCAAA       420
     AGGTTACTCC TATTTGGAAT TATAATAAAT TGTTTCGGGT CGGTAATTGG GTTTGTTGGC       480
     CATTCTTTCT TTTCCTTACT TATTATGGCT CGTTTTATTC AAGGGGCTGG TGCAGCTGCA       540
     TTTCCAGCAC TCGTAATGGT TGTAGTTGCG CGCTATATTC CAAAGGAAAA TAGGGGTAAA       600
     GCATTTGGTC TTATTGGATC GATAGTAGCC ATGGGAGAAG GAGTCGGTCC AGCGATTGGT       660
     GGAATGATAG CCCATTATAT TCATTGGTCC TATCTTCTAC TCATTCCTAT GATAACAATT       720
     ATCACTGTTC CGTTTCTTAT GAAATTATTA AAGAAAGAAG TAAGGATAAA AGGTCATTTT       780
     GATATCAAAG GAATTATACT AATGTCTGTA GGCATTGTAT TTTTTATGTT GTTTACAACA       840
     TCATATAGCA TTTCTTTTCT TATCGTTAGC GTGCTGTCAT TCCTGATATT TGTAAAACAT       900
     ATCAGGAAAG TAACAGATCC TTTTGTTGAT CCCGGATTAG GGAAAAATAT ACCTTTTATG       960
     ATTGGAGTTC TTTGTGGGGG AATTATATTT GGAACAGTAG CAGGGTTTGT CTCTATGGTT      1020
     CCTTATATGA TGAAAGATGT TCACCAGCTA AGTACTGCCG AAATCGGAAG TGTAATTATT      1080
     TTCCCTGGAA CAATGAGTGT CATTATTTTC GGCTACATTG GTGGGATACT TGTTGATAGA      1140
     AGAGGTCCTT TATACGTGTT AAACATCGGA GTTACATTTC TTTCTGTTAG CTTTTTAACT      1200
     GCTTCCTTTC TTTTAGAAAC AACATCATGG TTCATGACAA TTATAATCGT ATTTGTTTTA      1260
     GGTGGGCTTT CGTTCACCAA AACAGTTATA TCAACAATTG TTTCAAGTAG CTTGAAACAG      1320
     CAGGAAGCTG GTGCTGGAAT GAGTTTGCTT AACTTTACCA GCTTTTTATC AGAGGGAACA      1380
     GGTATTGCAA TTGTAGGTGG TTTATTATCC ATACCCTTAC TTGATCAAAG GTTGTTACCT      1440
     ATGGAAGTTG ATCAGTCAAC TTATCTGTAT AGTAATTTGT TATTACTTTT TTCAGGAATC      1500
     ATTGTCATTA GTTGGCTGGT TACCTTGAAT GTATATAAAC ATTCTCAAAG GGATTTCTAA      1560
     ATCGTTAAGG GATCAACTTT GGGAGAGAGT TCAAAATTGA TCCTTTTTTT ATAACAGGAA      1620
     TTCAAATCTT TTTGTTCCAT TAAA                                             1644
//