Class L (Bacillus subtilis - plasmid pJH1 - tet(L) gene)

ID   EF17153    standard; DNA; PRO; 2140 BP.
XX
AC   U17153;
XX
NI   g1041816
XX
DT   08-NOV-1995 (Rel. 45, Created)
DT   29-SEP-1996 (Rel. 49, Last updated, Version 2)
XX
DE   Enterococcus faecalis plasmid pjh1 tetracycline resistant (tetL)
DE   gene, complete cds.
XX
KW   .
XX
OS   Enterococcus faecalis
OC   Eubacteria; Firmicutes; Low G+C gram-positive bacteria; Enterococcus.
XX
OS   Plasmid pJH1
OC   plasmids.
XX
RN   [1]
RP   1-2140
RX   MEDLINE; 95354964.
RA   Platteeuw C., Michiels F., Joos H., Seurinck J., de Vos W.M.;
RT   "Characterization and heterologous expression of the tetL gene and
RT   identification of iso-ISS1 elements from Enterococcus faecalis plasmid
RT   pJH1";
RL   Gene 160:89-93(1995).
XX
RN   [2]
RP   1-2140
RA   Platteeuw C.;
RT   ;
RL   Submitted (15-NOV-1994) to the EMBL/GenBank/DDBJ databases.
RL   Christ Platteeuw, NIZO, Kernhemseweg ZB Ede, Netherlands
XX
DR   SPTREMBL; P75005; P75005.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2140
FT                   /organism="Enterococcus faecalis"
FT                   /strain="jh1"
FT   CDS             complement(<1..477)
FT                   /codon_start=1
FT                   /db_xref="PID:g1568598"
FT                   /db_xref="SPTREMBL:P75005"
FT                   /transl_table=11
FT                   /product="transposase"
FT                   /translation="MTTNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVS
FT                   HTTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
FT                   WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR"
FT   source          complement(1..477)
FT                   /organism="Plasmid pJH1"
FT                   /insertion_seq="iso-ISS1"
FT   CDS             701..2077
FT                   /codon_start=1
FT                   /db_xref="PID:g1041817"
FT                   /transl_table=11
FT                   /gene="tetL"
FT                   /product="tetracycline resistant protein"
FT                   /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
FT                   KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
FT                   LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
FT                   HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
FT                   ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
FT                   MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
FT                   SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
FT                   GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
FT                   "
XX
SQ   Sequence 2140 BP; 614 A; 352 C; 399 G; 775 T; 0 other;
     ACGATGTTCT GTCCCTTGAT AAAAGCCGTA TTCTTTTAGT TTCTTAAAGG CACTTGTAAT        60
     AGAGGGGGCT TTATCTGTGA CTACAACCTT CGGTTCATCA AACTGCTTCA CTAACCGCTT       120
     AAGAAAAGCA TAGGCTGCTT GTGTGTCCCG TTTTTTACGT AACCAAATAT CCAAGGTTAA       180
     ACCATCTGCA TCGATGGCTC GATACAAATA ATGCCATTTT CCTTTAATTT TGATGTACGT       240
     TTCATCCATT TTCCATGAAT AAAAGGATTT TTTATTTTTC TTTTTCCAAA TTTGATAGAG       300
     TAGTTTGCCA TATTCTTGCA CCCAACGATA AATCGTCGTA TGAGAAACGT TAATGCCACG       360
     ATCATATAAG ATTTCTTGAA CTTCACGATA GCTAAGGTTA TAACGAAGAT AGTAGCCCAC       420
     GGCTACAATA ATCACATCCT GCTGAAATTG CTTTCCTTTA AAATGATTCG TCGTCATTCC       480
     TCCTGCTATC TTTTTCTATT ATTCTACCTT ATTTGATAGT AGATTTAAAA CTTTGCAACA       540
     GAACCATTTA AAACTTAGTT TATATGTGGT AAAATGTTTT AATCAAGTTT AGGAGGAATT       600
     AATTATGAAG TGTAATTAAT GTAACAGGGT TCAATTAAAA GAGGGAAGCG TATCATTAAC       660
     CCTATAAACT ACGTCTGCCC TCATTATTGG AGGGTGAAAT GTGAATACAT CCTATTCACA       720
     ATCGAATTTA CGACACAACC AAATTTTAAT TTGGCTTTGC ATTTTATCTT TTTTTAGCGT       780
     ATTAAATGAA ATGGTTTTGA ACGTCTCATT ACCTGATATT GCAAATGATT TTAATAAACC       840
     ACCTGCGAGT ACAAACTGGG TGAACACAGC CTTTATGTTA ACCTTTTCCA TTGGAACAGC       900
     TGTATATGGA AAGCTATCTG ATCAATTAGG CATCAAAAGG TTACTCCTAT TTGGAATTAT       960
     AATAAATTGT TTCGGGTCGG TAATTGGGTT TGTTGGCCAT TCTTTCTTTT CCTTACTTAT      1020
     TATGGCTCGT TTTATTCAAG GGGCTGGTGC AGCTGCATTT CCAGCACTCG TAATGGTTGT      1080
     AGTTGCGCGC TATATTCCAA AGGAAAATAG GGGTAAAGCA TTTGGTCTTA TTGGATCGAT      1140
     AGTAGCCATG GGAGAAGGAG TCGGTCCAGC GATTGGTGGA ATGATAGCCC ATTATATTCA      1200
     TTGGTCCTAT CTTCTACTCA TTCCTATGAT AACAATTATC ACTGTTCCGT TTCTTATGAA      1260
     ATTATTAAAG AAAGAAGTAA GGATAAAAGG TCATTTTGAT ATCAAAGGAA TTATACTAAT      1320
     GTCTGTAGGC ATTGTATTTT TTATGTTGTT TACAACATCA TATAGCATTT CTTTTCTTAT      1380
     CGTTAGCGTG CTGTCATTCC TGATATTTGT AAAACATATC AGGAAAGTAA CAGATCCTTT      1440
     TGTTGATCCC GGATTAGGGA AAAATATACC TTTTATGATT GGAGTTCTTT GTGGGGGAAT      1500
     TATATTTGGA ACAGTAGCAG GGTTTGTCTC TATGGTTCCT TATATGATGA AAGATGTTCA      1560
     CCAGCTAAGT ACTGCCGAAA TCGGAAGTGT AATTATTTTC CCTGGAACAA TGAGTGTCAT      1620
     TATTTTCGGC TACATTGGTG GGATACTTGT TGATAGAAGA GGTCCTTTAT ACGTGTTAAA      1680
     CATCGGAGTT ACATTTCTTT CTGTTAGCTT TTTAACTGCT TCCTTTCTTT TAGAAACAAC      1740
     ATCATGGTTC ATGACAATTA TAATCGTATT TGTTTTAGGT GGGCTTTCGT TCACCAAAAC      1800
     AGTTATATCA ACAATTGTTT CAAGTAGCTT GAAACAGCAG GAAGCTGGTG CTGGAATGAG      1860
     TTTGCTTAAC TTTACCAGCT TTTTATCAGA GGGAACAGGT ATTGCAATTG TAGGTGGTTT      1920
     ATTATCCATA CCCTTACTTG ATCAAAGGTT GTTACCTATG GAAGTTGATC AGTCAACTTA      1980
     TCTGTATAGT AATTTGTTAT TACTTTTTTC AGGAATCATT GTCATTAGTT GGCTGGTTAC      2040
     CTTGAATGTA TATAAACATT CTCAAAGGGA TTTCTAAATC GTTAAGGGAT CAACTTTGGG      2100
     AGAGAGTTCA AAATTGATCC TTTTTTTATA ACAGGAATTC                            2140
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