Class L (Bacillus subtilis - plasmid pNS1981 - tet(L) gene)

ID   PP981TET   standard; DNA; PRO; 1628 BP.
XX
AC   D00006; N00006;
XX
NI   g216835
XX
DT   21-APR-1990 (Rel. 23, Created)
DT   14-MAY-1997 (Rel. 51, Last updated, Version 3)
XX
DE   Bacillus subtilis plasmid pNS1981 tetracycline resistance gene
DE   (tet) and flanking region, complete cds.
XX
KW   bacterial cell membrane binding protein; gene; mesophilic; plasmid; tet;
KW   TET protein; tetracycline resistance.
XX
OS   Bacillus subtilis
OC   Eubacteria; Firmicutes; Low G+C gram-positive bacteria; Bacillaceae;
OC   Bacillus.
XX
OG   Plasmid pNS1981
XX
RN   [1]
RP   1-1628
RX   MEDLINE; 86031344.
RA   Hoshino T., Ikeda T., Tomizuka N., Furukawa K.;
RT   "Nucleotide sequence of the tetracycline resistance gene of pTHT15, a
RT   thermophilic Bacillus plasmid: comparison with staphylococcal Tcr
RT   controls";
RL   Gene 37:131-138(1985).
XX
RN   [2]
RP   1-1628
RA   Sakaguchi R., Shishido K., Hoshino T., Furukawa K.;
RT   "The nucleotide sequence of the tetracycline resistance gene of plasmid
RT   pNS1981 from Bacillus subtilis differs from pTHT15 from a thermophilic
RT   Bacillus by two base pairs";
RL   Plasmid 16:72-73(1986).
XX
DR   SPTREMBL; P72219; P72219.
DR   SWISS-PROT; P05658; LPTR_BACST.
XX
CC   The nucleotide sequence of pNS1981 is identical to that of pTHT15
CC   except for only two consecutive bases (position 1275-1276) within
CC   the Tcr structural gene (tet).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1628
FT                   /organism="Bacillus subtilis"
FT                   /sequenced_mol="DNA"
FT                   /plasmid="Plasmid pNS1981"
FT                   /strain="Marburg"
FT   misc_feature    69
FT                   /note="tet protein mRNA start site"
FT   CDS             93..155
FT                   /codon_start=1
FT                   /db_xref="PID:g216836"
FT                   /db_xref="SWISS-PROT:P05658"
FT                   /transl_table=11
FT                   /product="leader peptide"
FT                   /translation="MKCNECNRVQLKEGSVSLTL"
FT   CDS             189..1565
FT                   /codon_start=1
FT                   /db_xref="PID:d1000431"
FT                   /db_xref="SPTREMBL:P72219"
FT                   /transl_table=11
FT                   /gene="tet"
FT                   /product="tet protein"
FT                   /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
FT                   KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
FT                   LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
FT                   HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
FT                   ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
FT                   MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
FT                   SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
FT                   GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
FT                   "
XX
SQ   Sequence 1628 BP; 471 A; 243 C; 323 G; 591 T; 0 other;
     GGCCATATTG TTGTATAAGT GATGAAATAC TGAATTTAAA ACTTAGTTTA TATGTGGTAA        60
     AATGTTTTAA TCAAGTTTAG GAGGAATTAA TTATGAAGTG TAATGAATGT AACAGGGTTC       120
     AATTAAAAGA GGGAAGCGTA TCATTAACCC TATAAACTAC GTCTGCCCTC ATTATTGGAG       180
     GGTGAAATGT GAATACATCC TATTCACAAT CGAATTTACG ACACAACCAA ATTTTAATTT       240
     GGCTTTGCAT TTTATCTTTT TTTAGCGTAT TAAATGAAAT GGTTTTGAAC GTCTCATTAC       300
     CTGATATTGC AAATGATTTT AATAAACCAC CTGCGAGTAC AAACTGGGTG AACACAGCCT       360
     TTATGTTAAC CTTTTCCATT GGAACAGCTG TATATGGAAA GCTATCTGAT CAATTAGGCA       420
     TCAAAAGGTT ACTCCTATTT GGAATTATAA TAAATTGTTT CGGGTCGGTA ATTGGGTTTG       480
     TTGGCCATTC TTTCTTTTCC TTACTTATTA TGGCTCGTTT TATTCAAGGG GCTGGTGCAG       540
     CTGCATTTCC AGCACTCGTA ATGGTTGTAG TTGCGCGCTA TATTCCAAAG GAAAATAGGG       600
     GTAAAGCATT TGGTCTTATT GGATCGATAG TAGCCATGGG AGAAGGAGTC GGTCCAGCGA       660
     TTGGTGGAAT GATAGCCCAT TATATTCATT GGTCCTATCT TCTACTCATT CCTATGATAA       720
     CAATTATCAC TGTTCCGTTT CTTATGAAAT TATTAAAGAA AGAAGTAAGG ATAAAAGGTC       780
     ATTTTGATAT CAAAGGAATT ATACTAATGT CTGTAGGCAT TGTATTTTTT ATGTTGTTTA       840
     CAACATCATA TAGCATTTCT TTTCTTATCG TTAGCGTGCT GTCATTCCTG ATATTTGTAA       900
     AACATATCAG GAAAGTAACA GATCCTTTTG TTGATCCCGG ATTAGGGAAA AATATACCTT       960
     TTATGATTGG AGTTCTTTGT GGGGGAATTA TATTTGGAAC AGTAGCAGGG TTTGTCTCTA      1020
     TGGTTCCTTA TATGATGAAA GATGTTCACC AGCTAAGTAC TGCCGAAATC GGAAGTGTAA      1080
     TTATTTTCCC TGGAACAATG AGTGTCATTA TTTTCGGCTA CATTGGTGGG ATACTTGTTG      1140
     ATAGAAGAGG TCCTTTATAC GTGTTAAACA TCGGAGTTAC ATTTCTTTCT GTTAGCTTTT      1200
     TAACTGCTTC CTTTCTTTTA GAAACAACAT CATGGTTCAT GACAATTATA ATCGTATTTG      1260
     TTTTAGGTGG GCTTTCGTTC ACCAAAACAG TTATATCAAC AATTGTTTCA AGTAGCTTGA      1320
     AACAGCAGGA AGCTGGTGCT GGAATGAGTT TGCTTAACTT TACCAGCTTT TTATCAGAGG      1380
     GAACAGGTAT TGCAATTGTA GGTGGTTTAT TATCCATACC CTTACTTGAT CAAAGGTTAT      1440
     TACCTATGGA AGTTGATCAG TCAACTTATC TGTATAGTAA TTTGTTATTA CTTTTTTCAG      1500
     GAATCATTGT CATTAGTTGG CTGGTTACCT TGAATGTATA TAAACATTCT CAAAGGGATT      1560
     TCTAAATCGT TAAGGGATCA ACTTTGGGAG AGAGTTCAAA ATTGATCCTT TTTTTATAAC      1620
     AGGAATTC                                                               1628
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