Class L (Bacillus stearothermophilus - plasmid pTHT15 - tet(L) gene)

ID   BSTHT15    standard; DNA; PRO; 1628 BP.
XX
AC   M11036;
XX
NI   g143737
XX
DT   02-JUL-1986 (Rel. 09, Created)
DT   21-APR-1990 (Rel. 23, Last updated, Version 1)
XX
DE   B.stearothermophilus plasmid pTHT15 tetracycline resistance gene,
DE   complete cds.
XX
KW   attenuator; drug resistance; TET protein; tetracycline resistance;
KW   translational control.
XX
OS   Bacillus stearothermophilus
OC   Eubacteria; Firmicutes; Low G+C gram-positive bacteria;
OC   Bacillaceae; Bacillus.
XX
OG   Plasmid pTHT15
XX
RN   [1]
RP   1-1628
RX   MEDLINE; 86031344.
RA   Hoshino T., Ikeda T., Tomizuka N., Furukawa K.;
RT   "Nucleotide sequence of the tetracycline resistance gene of
RT   pTHT15, a thermophilic Bacillus plasmid: comparison with
RT   staphylococcal Tcr controls";
RL   Gene 37:131-138(1985).
XX
DR   SWISS-PROT; P05658; LPTR_BACST.
DR   SWISS-PROT; P07561; TCR_BACST.
XX
CC   Draft entry and clean copy sequence for [1] kindly provided by
CC   T.Hoshino, 13-JAN-1986. The presence of three inverted repeats
CC   (positions 114-123 and 143-151; 129-140 and 158-170; 164-170 and
CC   178-184), which can form two different conformations of the mRNA,
CC   suggests that the TC-R gene is regulated by a translational
CC   attenuation mechanism. Potential -35 and -10 regions are located at
CC   positions 35-40 and 58-63 respectively and ribosome binding sites
CC   at 81-84 (leader) and 177-184 (TC-R). A rho-independent terminator
CC   structure is found at positions 1571-1610. The precise 3' end of
CC   the mRNA is not known.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1628
FT                   /organism="Bacillus stearothermophilus"
FT                   /plasmid="Plasmid pTHT15"
FT   mRNA            69..>1565
FT                   /note="TET mRNA (5' end approx.)"
FT   CDS             93..155
FT                   /note="leader peptide"
FT                   /db_xref="PID:g143738"
FT                   /db_xref="SWISS-PROT:P05658"
FT                   /translation="MKCNECNRVQLKEGSVSLTL"
FT   CDS             189..1565
FT                   /note="tetracycline resistance protein (gtg start codon)"
FT                   /db_xref="PID:g143739"
FT                   /db_xref="SWISS-PROT:P07561"
FT                   /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
FT                   KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
FT                   LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
FT                   HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
FT                   ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
FT                   MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
FT                   SFLLETTSWFMTIIIVFVLGGLLFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
FT                   GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
FT                   "
XX
SQ   Sequence 1628 BP; 471 A; 243 C; 323 G; 591 T; 0 other;
     GGCCATATTG TTGTATAAGT GATGAAATAC TGAATTTAAA ACTTAGTTTA TATGTGGTAA        60
     AATGTTTTAA TCAAGTTTAG GAGGAATTAA TTATGAAGTG TAATGAATGT AACAGGGTTC       120
     AATTAAAAGA GGGAAGCGTA TCATTAACCC TATAAACTAC GTCTGCCCTC ATTATTGGAG       180
     GGTGAAATGT GAATACATCC TATTCACAAT CGAATTTACG ACACAACCAA ATTTTAATTT       240
     GGCTTTGCAT TTTATCTTTT TTTAGCGTAT TAAATGAAAT GGTTTTGAAC GTCTCATTAC       300
     CTGATATTGC AAATGATTTT AATAAACCAC CTGCGAGTAC AAACTGGGTG AACACAGCCT       360
     TTATGTTAAC CTTTTCCATT GGAACAGCTG TATATGGAAA GCTATCTGAT CAATTAGGCA       420
     TCAAAAGGTT ACTCCTATTT GGAATTATAA TAAATTGTTT CGGGTCGGTA ATTGGGTTTG       480
     TTGGCCATTC TTTCTTTTCC TTACTTATTA TGGCTCGTTT TATTCAAGGG GCTGGTGCAG       540
     CTGCATTTCC AGCACTCGTA ATGGTTGTAG TTGCGCGCTA TATTCCAAAG GAAAATAGGG       600
     GTAAAGCATT TGGTCTTATT GGATCGATAG TAGCCATGGG AGAAGGAGTC GGTCCAGCGA       660
     TTGGTGGAAT GATAGCCCAT TATATTCATT GGTCCTATCT TCTACTCATT CCTATGATAA       720
     CAATTATCAC TGTTCCGTTT CTTATGAAAT TATTAAAGAA AGAAGTAAGG ATAAAAGGTC       780
     ATTTTGATAT CAAAGGAATT ATACTAATGT CTGTAGGCAT TGTATTTTTT ATGTTGTTTA       840
     CAACATCATA TAGCATTTCT TTTCTTATCG TTAGCGTGCT GTCATTCCTG ATATTTGTAA       900
     AACATATCAG GAAAGTAACA GATCCTTTTG TTGATCCCGG ATTAGGGAAA AATATACCTT       960
     TTATGATTGG AGTTCTTTGT GGGGGAATTA TATTTGGAAC AGTAGCAGGG TTTGTCTCTA      1020
     TGGTTCCTTA TATGATGAAA GATGTTCACC AGCTAAGTAC TGCCGAAATC GGAAGTGTAA      1080
     TTATTTTCCC TGGAACAATG AGTGTCATTA TTTTCGGCTA CATTGGTGGG ATACTTGTTG      1140
     ATAGAAGAGG TCCTTTATAC GTGTTAAACA TCGGAGTTAC ATTTCTTTCT GTTAGCTTTT      1200
     TAACTGCTTC CTTTCTTTTA GAAACAACAT CATGGTTCAT GACAATTATA ATCGTATTTG      1260
     TTTTAGGTGG GCTTCTGTTC ACCAAAACAG TTATATCAAC AATTGTTTCA AGTAGCTTGA      1320
     AACAGCAGGA AGCTGGTGCT GGAATGAGTT TGCTTAACTT TACCAGCTTT TTATCAGAGG      1380
     GAACAGGTAT TGCAATTGTA GGTGGTTTAT TATCCATACC CTTACTTGAT CAAAGGTTAT      1440
     TACCTATGGA AGTTGATCAG TCAACTTATC TGTATAGTAA TTTGTTATTA CTTTTTTCAG      1500
     GAATCATTGT CATTAGTTGG CTGGTTACCT TGAATGTATA TAAACATTCT CAAAGGGATT      1560
     TCTAAATCGT TAAGGGATCA ACTTTGGGAG AGAGTTCAAA ATTGATCCTT TTTTTATAAC      1620
     AGGAATTC                                                               1628
//