napB gene; napC gene; regulatory protein; tetracycline efflux from Enterococcus hirae

ID   EHNAPBC    standard; DNA; PRO; 2000 BP.
XX
AC   AJ000346;
XX
SV   AJ000346.1
XX
NI   e1055721
XX
DT   22-JUL-1997 (Rel. 52, Created)
DT   22-JUL-1997 (Rel. 52, Last updated, Version 1)
XX
DE   Enterococcus hirae napB and napC genes
XX
KW   napB gene; napC gene; regulatory protein; tetracycline efflux.
XX
OS   Enterococcus hirae
OC   Bacteria; Firmicutes; Bacillus/Clostridium group; Enterococcaceae;
OC   Enterococcus.
OG   Plasmid pCX12
XX
RN   [1]
RP   1-2000
RA   Solioz M.;
RT   ;
RL   Submitted (10-JUL-1997) to the EMBL/GenBank/DDBJ databases.
RL   Solioz M., Clinical Pharmacology, Universtity of Berne, Murtenstrasse
RL   35, 3010 Berne, SWITZERLAND.
XX
RN   [2]
RA   Solioz M., Strausak D.;
RT   "A putative tetracyclin efflux system of Enterococcus hirae";
RL   Unpublished.
XX
DR   SPTREMBL; O32602; O32602.
DR   SPTREMBL; O32603; O32603.
XX
CC   Related sequences M81961, inverted X68646
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2000
FT                   /organism="Enterococcus hirae"
FT                   /db_xref="taxon:1354"
FT                   /sequenced_mol="DNA"
FT                   /clone="pCX12"
FT                   /clone_lib="pCX"
FT                   /plasmid="pCX12"
FT                   /strain="ATCC9790"
FT   RBS             116..122
FT                   /gene="napB"
FT   CDS             129..581
FT                   /codon_start=1
FT                   /db_xref="PID:e328772"
FT                   /db_xref="SPTREMBL:O32602"
FT                   /transl_table=11
FT                   /gene="napB"
FT                   /product="NapB protein"
FT                   /function="putative regulatory protein"
FT                   /protein_id="CAA04020.1"
FT                   /translation="MDSRLKLISEMVDLTNEITWRNKPKLEQVLEGYSLNEIDLIEKVA
FT                   SIPNPNVTKLANASYMTRGAISKLTKKMITNGLLESYQKPENKKERYFLLTAEGQRINK
FT                   MHQKLHEEFLKENEVVFQQMTKEEFDTVFRFIGRFRENLKRIKLEK"
FT   RBS             745..748
FT                   /gene="napC"
FT   CDS             756..1958
FT                   /codon_start=1
FT                   /db_xref="PID:e328773"
FT                   /db_xref="SPTREMBL:O32603"
FT                   /note="Telve predicted transmembranous helices"
FT                   /transl_table=11
FT                   /gene="napC"
FT                   /product="NapC protein"
FT                   /function="putative tetracyclin efflux protein"
FT                   /protein_id="CAA04021.1"
FT                   /translation="MNTTINKHALVFGFTSVFLIGLGLTIVNPVIPFMVEQYTKNTQQQ
FT                   ATTVTLLSAIYAFSMFLAAPMLGALSDRFGRKIILISSLFGSAIGYYLFGFGGALWILF
FT                   LGRIIEGLTGGEISAILAYFADLTPIESRTKYFGWISATVGIGTAAGPLIGGFLAQYGP
FT                   AIPLYVASFLSLSNAVYGYFFMPESLTKRERTRNLSLQQINPFKQLQLVFTFRSVKWLL
FT                   ITGFLIWLPNGSFQAIFAQFSIDTFHLSPIIIGFTFSLIGIMDIFAQLLIMPILLKFWR
FT                   ENQIITMGITSEMIGYSVIILSAFYGSIPCFIIGMVFFGLGDAIFSPSYNGLISTYASK
FT                   EDQGKIQGASQSIQALARVIGPMIGGQLYANFHHTMPFIIGFILLGLATFIVKPKVKRS
FT                   P"
XX
SQ   Sequence 2000 BP; 598 A; 366 C; 373 G; 663 T; 0 other;
     ACTCTGATCT CACCTCTGAT TCTTAAATAT TTCACTAAAA AAGTATACGC CAATTAACTT        60
     ACTATCTATC AATGAATGTT GTGATGATAA TGTCCATTCG TTATACTAGA GAAAAAGGAC       120
     GGTGAAAAAT GGACTCAAGA CTTAAATTGA TTAGTGAGAT GGTCGATCTG ACTAACGAGA       180
     TCACTTGGCG TAATAAACCA AAATTAGAAC AAGTACTCGA AGGCTATTCT TTAAATGAGA       240
     TCGACTTAAT CGAAAAAGTA GCATCAATTC CAAATCCAAA TGTAACCAAA TTAGCAAATG       300
     CTAGTTACAT GACTCGTGGA GCGATTAGTA AGCTAACTAA AAAAATGATC ACTAACGGCT       360
     TACTTGAAAG CTATCAGAAA CCTGAAAATA AAAAAGAGCG TTACTTTCTC CTGACAGCTG       420
     AAGGTCAGCG TATTAACAAA ATGCATCAAA AGCTCCATGA AGAATTTCTT AAAGAAAATG       480
     AGGTCGTTTT TCAACAAATG ACTAAGGAAG AGTTTGATAC TGTTTTTCGT TTTATCGGTC       540
     GCTTTAGAGA AAATTTAAAA AGAATAAAAC TAGAAAAATA AAGTGCATGT GTAATCAACC       600
     GTGATCTTAA GATCACGTTG ATTTTTTGCT CTCTTCTTCG TTAGATTTCA TTCTTCTATC       660
     TTAGTATCTC TCCGTTTATT GTTGACAAGG AAACAATAAA CAGATACTAT ATAAAATGTT       720
     TCCTAGGAAA CATTTTATAG CTAAGGAGTT TTGATATGAA TACAACAATC AACAAACATG       780
     CACTCGTTTT TGGTTTCACT TCGGTCTTTT TGATTGGATT AGGGTTAACA ATCGTTAATC       840
     CGGTCATCCC ATTTATGGTA GAGCAATACA CAAAAAACAC ACAGCAACAA GCAACCACGG       900
     TGACACTACT CAGTGCGATC TATGCTTTTT CCATGTTTTT AGCCGCCCCT ATGTTAGGCG       960
     CACTCAGTGA TCGGTTTGGT CGGAAAATTA TTTTGATCAG TAGTTTGTTT GGCTCTGCGA      1020
     TTGGGTACTA TTTATTTGGC TTTGGTGGAG CTTTATGGAT ACTTTTTCTT GGTAGAATAA      1080
     TTGAGGGGCT GACCGGCGGC GAAATCTCAG CTATTCTTGC CTATTTTGCT GATTTGACGC      1140
     CAATTGAAAG TCGAACAAAA TATTTTGGAT GGATTAGCGC GACGGTTGGG ATTGGAACTG      1200
     CAGCAGGTCC TTTGATTGGT GGGTTCTTGG CACAGTACGG ACCAGCAATT CCCTTATACG      1260
     TTGCCTCCTT TTTGTCTTTG AGTAATGCGG TTTACGGTTA TTTTTTCATG CCTGAAAGTT      1320
     TGACTAAACG TGAACGTACA AGAAATCTTT CTCTTCAGCA AATCAATCCC TTCAAGCAAC      1380
     TCCAATTGGT TTTCACTTTT CGATCAGTCA AATGGTTATT GATCACAGGT TTTTTGATTT      1440
     GGCTACCCAA CGGTTCATTC CAAGCGATTT TTGCTCAATT TTCAATTGAT ACTTTCCATC      1500
     TATCGCCAAT TATCATCGGT TTTACTTTCT CGTTAATTGG CATTATGGAC ATTTTTGCTC      1560
     AACTGTTGAT TATGCCGATT TTATTAAAAT TTTGGCGAGA AAACCAGATC ATTACTATGG      1620
     GTATTACCAG TGAAATGATT GGTTATTCTG TCATCATCCT TTCCGCTTTT TATGGCTCGA      1680
     TCCCCTGCTT TATCATCGGC ATGGTCTTTT TCGGGCTAGG TGATGCGATT TTTAGTCCTT      1740
     CCTATAATGG TTTGATTTCA ACGTATGCTA GCAAAGAGGA CCAAGGGAAA ATCCAAGGGG      1800
     CTTCACAAAG CATCCAAGCA TTAGCTAGAG TGATCGGTCC GATGATCGGT GGTCAACTAT      1860
     ATGCTAATTT CCATCATACG ATGCCATTCA TTATTGGATT CATTTTATTA GGTTTAGCTA      1920
     CTTTTATCGT TAAACCAAAG GTAAAAAGAT CCCCCTAAAG CATCCCTATA TAAAAAAACT      1980
     AATCGAGTGA CTGTAGTATT                                                  2000
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