Class V (Mycobacterium smegmatis tet(V) gene)
ID AF030344 standard; DNA; PRO; 2224 BP.
XX
AC AF030344;
XX
NI g2613095
XX
DT 15-NOV-1997 (Rel. 53, Created)
DT 15-NOV-1997 (Rel. 53, Last updated, Version 1)
XX
DE Mycobacterium smegmatis tetracycline-resistance determinant tetV
DE (tetV) gene, complete cds.
XX
KW .
XX
OS Mycobacterium smegmatis
OC Eubacteria; Firmicutes; Actinomycetes; Mycobacteria; Mycobacteriaceae;
OC Mycobacterium.
XX
RN [1]
RP 1-2224
RA De Rossi E., Blokpoel M.C.J., Cantoni R., Branzoni M., Riccardi G.,
RA Young D.B., De Smet K.A.L., Ciferri O.;
RT "Molecular cloning and functional analysis of a novel
RT tetracycline-resistance determinant, tetV, from Mycobacterium
RT smegmatis";
RL Unpublished.
XX
RN [2]
RP 1-2224
RA De Rossi E.;
RT ;
RL Submitted (19-OCT-1997) to the EMBL/GenBank/DDBJ databases.
RL Genetica e Microbiologia, University of Pavia, Abbiategrasso 207, Pavia
RL 27100, Italy
XX
DR SPTREMBL; O31137; O31137.
XX
FH Key Location/Qualifiers
FH
FT source 1..2224
FT /organism="Mycobacterium smegmatis"
FT /strain="mc2155"
FT CDS complement(462..1721)
FT /codon_start=1
FT /db_xref="PID:g2613096"
FT /db_xref="SPTREMBL:O31137"
FT /note="tetracycline-efflux protein; integral membrane
FT protein; member of the Major Facilitator Superfamily of
FT antibiotic efflux proteins"
FT /transl_table=11
FT /gene="tetV"
FT /function="resistance to tetracycline by an active
FT tetracycline efflux; energy-dependent process that
FT decreases the accumulation of the antibiotic in whole
FT cells"
FT /product="tetracycline-resistance determinant tetV"
FT /translation="MRSPRPVAGWRVLAPFRIREYRLLIAAVTLSIFAEGMWSVVMALQ
FT VIAIDNDPASLSLVATCLGVGLVAFVLVGGITADRINQRTIIIAVEVVNFVTVAVISAL
FT ALLGVLKIWHMAVAAGILGIAAAFFFPAYSAILPRILPPEQLLAANGVEGVVRPVFQRS
FT VGPAVAGMVIGATMPSIGAVVVAVLFALGLALLVATRPPAQPASEHHERPHVLRDLREG
FT FAFVLKTPWLLWTVLFASMFVLVVLGPIEVLLPFIAQDRFADGARAYGFILAFFGIGSA
FT MGALTVSSRRMPRRYLTTMMLMWGLGSIPLVIVGYTSSFPLMAAATFVIGVTDGAGMVI
FT WGTLLQRRVPTEMLGRVSSLDFFVSLAFMPLSFAIVGPLSKVVSMEVIFATAGLVPVAI
FT AAVAFTAARMHRDEVANPLL"
XX
SQ Sequence 2224 BP; 415 A; 742 C; 723 G; 344 T; 0 other;
GGTACCTGCA TGTCCGTGGG CGGGACCGGC GCCCCGCGCT TGCCGGTGCC GTCGTCGGTA 60
TCACGCTCGC GGGTGTGTTC GTCGTCGGCG CCCTCATTGC CCGCGAGATC CCCGGCATCC 120
GTGAATTCAT CACGCGTGTA CTGGAATTCA GCAATACCGG GCCGCTGGCG TTGATCGTGT 180
TCATCACCGT CATCAACGGC GTCGCCGAGG AACTGTTCTT CCGCGGTGCG CTGTACTCCG 240
CGCTCGGGAA GTTCTACCCG GTGGTGGTGT CGACGCTGAT CTACATCGTG ACCACGATGG 300
CCTCGGGCAA CCCCATGCTG GGGTTCGCGG CCATCATCCT GGGCGCGGTG TGCGCCATGG 360
AACGCCGCGT TACCGGGGGT GTGCTCGCAC CGATGCTCAC CCATTTCTTC TGGGGTCTGG 420
CGATGGTGCT CGCACTCCCG CCGATCTTCG GCGTGTAGAC GTCACAGCAG TGGGTTCGCC 480
ACCTCGTCAC GGTGCATGCG CGCCGCGGTG AACGCCACGG CCGCGATCGC CACGGGCACC 540
AGACCCGCCG TCGCGAAGAT CACCTCCATC GAGACCACCT TCGACAGCGG ACCCACGATC 600
GCGAATGACA ACGGCATGAA CGCCAGCGAT ACGAAGAAGT CCAGGCTCGA CACGCGGCCC 660
AGCATCTCGG TGGGCACACG CCGTTGCAGC AGCGTTCCCC AGATCACCAT GCCAGCGCCG 720
TCGGTGACGC CGATGACGAA CGTCGCAGCG GCCATCAGCG GGAACGACGA TGTATATCCC 780
ACGATCACAA GGGGAATCGA GCCGAGACCC CACATCAGCA TCATGGTCGT GAGATAGCGG 840
CGCGGCATGC GCCGCGACGA CACCGTCAGC GCGCCCATCG CACTGCCGAT ACCGAAGAAC 900
GCCAGGATGA AACCGTAGGC GCGGGCGCCG TCGGCGAAGC GGTCCTGTGC GATGAACGGC 960
AGCAGCACCT CGATGGGTCC CAGCACGACG AGCACGAACA TGCTCGCGAA CAGCACGGTC 1020
CACAGCAGCC ACGGTGTCTT CAGGACGAAG GCGAAACCTT CACGCAGGTC CCGCAATACG 1080
TGCGGGCGCT CATGGTGCTC GGAGGCGGGC TGGGCGGGCG GACGGGTCGC GACCAGCAGC 1140
GCCAGGCCGA GCGCGAACAG CACCGCCACC ACGACCGCGC CGATCGACGG CATCGTTGCA 1200
CCGATGACCA TGCCGGCCAC CGCGGGGCCC ACCGAACGCT GGAACACCGG GCGTACCACG 1260
CCCTCGACAC CGTTGGCGGC CAGCAGCTGT TCGGGCGGCA GGATGCGCGG CAGGATCGCG 1320
CTGTAGGCCG GGAAGAAGAA CGCCGCCGCG ATGCCGAGAA TGCCTGCGGC AACGGCCATG 1380
TGCCAGATCT TCAGCACGCC CAGCAGGGCC AGCGCGGAGA TCACCGCGAC CGTGACGAAG 1440
TTGACCACCT CGACGGCAAT GATGATGGTG CGCTGGTTGA TCCGGTCCGC GGTGATGCCG 1500
CCGACGAGGA CGAACGCGAC CAGGCCGACA CCGAGGCACG TCGCGACCAG CGACAGTGAC 1560
GCCGGATCGT TGTCGATCGC GATCACCTGC AGCGCCATGA CCACAGACCA CATGCCCTCG 1620
GCGAAGATCG ACAGCGTGAC CGCGGCGATC AGCAGGCGGT ACTCGCGGAT CCGGAACGGT 1680
GCGAGTACGC GCCAGCCTGC GACCGGACGC GGCGAGCGCA CCGGTTCTTC GATGTCATGT 1740
TGCGTACTCA CAACATTTGA TGGTCCCAAC CTGCGGCGGC CGAGTCCAAT GAATTACGCC 1800
CGGGTGGTTG TCGGTGCCCT GGCCGATCAT CGGGCCATGA CAGACACCGA ACCGCAGTGG 1860
ACGTACCGCG CGCAGTGGTC CCCGGAATAC CGCGAATACT TCGGCCTGTG CCTGGAGTTC 1920
CCGACGCTGT GCGCGCGGGC GTTCACCGCG CACGAGGCCG TCGAACGTGC GCAGCGCCTC 1980
GTCGAGCAGG AGGTCGCCGA CAGGCTCGCG GCTGACATGG ATCTACCGCA GCCACTGACC 2040
GACCGGCGCT ACAGCGGCAG CTTCATGGTG CGCACCTCAC CCATTCTGCA CGCCAAGCTC 2100
ATGGTCGAAG CCGCCGAACA GCGGGTGTCG CTGAACCAGT GGGTGGTGCA GAAATTGGCA 2160
GCGCGTCCGA TGGTGTCGTT TGACGACTAC TAGGTGTCGG GCGTTGCTTG TGAACCCTGA 2220
TATC 2224
//