Class W (Butyrivibrio fibrisolvens tet(W) gene)
ID BFI222769 standard; DNA; PRO; 2387 BP.
XX
AC AJ222769;
XX
NI e1249305
XX
DT 03-FEB-1998 (Rel. 54, Created)
DT 16-OCT-1998 (Rel. 57, Last updated, Version 3)
XX
DE Butyrivibrio fibrisolvens tetW gene
XX
KW tetW gene.
XX
OS Butyrivibrio fibrisolvens
OC Bacteria; Firmicutes; Bacillus/Clostridium group; Clostridiaceae;
OC Butyrivibrio.
XX
RN [2]
RC (sites)
RA Scott K.P., Barbosa T.M., Forbes K.J., Flint H.J.;
RT "High frequency transfer of a naturally occurring chromosomal
RT tetracycline resistance element in the ruminal anaerobe Butyrivibrio
RT fibrisolvens";
RL Appl. Environ. Microbiol. 63:3405-3411(1997).
XX
RN [3]
RP 1-2387
RA Flint H.J.;
RT ;
RL Submitted (05-DEC-1997) to the EMBL/GenBank/DDBJ databases.
RL Flint H. J., Rowett Research Institute, Greenburn Road, Bucksburn,
RL Aberdeen, United Kingdom, AB21 9SB
XX
DR SPTREMBL; O52836; O52836.
XX
FH Key Location/Qualifiers
FH
FT source 1..2387
FT /organism="Butyrivibrio fibrisolvens"
FT /strain="1.230"
FT /clone="pTB1230 and pUTetVup3"
FT /clone_lib="lambda ZapII"
FT CDS 192..2111
FT /codon_start=1
FT /db_xref="PID:e1249306"
FT /db_xref="SPTREMBL:O52836"
FT /note="New ribosome protection determinant, approximately
FT 70% amino acid identity to TetO."
FT /function="tetracycline resistance"
FT /product="TetW protein"
FT /gene="tetW"
FT /translation="MKIINIGILAHVDAGKTTLTESLLYASGAISEPGSVEKGTTRTDT
FT MFLERQRGITIQAAVTSFQWHRCKVNIVDTPGHMDFLAEVYRSLAVLDGAILVISAKDG
FT VQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSLSPE
FT IVLEENTDIEAWDAVIENNDELLEKYIAGEPISREKLAREEQQRVQDASLFPVYHGSAK
FT NGLGIQPLMDAVTGLFQPIGEQGGAALCGSVFKVEYTDCGQRRVYLRLYSGTLRLRDTV
FT ALAGREKLKITEMRIPSKGEIVRTDTAYQGEIVILPSDSVRLNDVLGDQTRLPRKRWRE
FT DPLPMLRTTIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLGRVQLEVV
FT SALLSEKYKLETVVKEPSVIYMERPLKAASHTIHIEVPPNPFWASIGLSVTPLSLGSGV
FT QYESRVSLGYLNQSFQNAVRDGIRYGLEQGLFGWNVTDCKICFEYGLYYSPVSTPADFR
FT SLAPIVLEQALKESGTQLLEPYLSFILYAPQEYLSRAYHDAPKYCATIETAQVKKDEVV
FT FTGEIPARCIQAYRTDLAFYTNGRSVCLTELKGYQAAVGQPVIQPRRPNSRLDKVRHMF
FT QKVM"
XX
SQ Sequence 2387 BP; 604 A; 564 C; 637 G; 582 T; 0 other;
GGGCTTAGTT TTTTGTACCC AATTTAAGAA TACTTTTGCC TTATCAATTT TGACATATCC 60
CCAAAAACAG CAATCACAAA CAGGTGTATG CTGTATATGT GTATGTCCGC AACTTATAAT 120
CCCCAGTGGT AAAAGTATTT TACTGCTGGG GATTTTTATG CCCTTTGGGG CTGTAAAGGG 180
AGGACAATCA CATGAAAATA ATCAATATTG GAATTCTTGC CCATGTAGAC GCTGGAAAGA 240
CGACCTTGAC GGAGAGCCTG CTATATGCCA GCGGAGCCAT TTCAGAACCG GGGAGCGTCG 300
AAAAAGGGAC AACGAGGACG GACACCATGT TTTTGGAGCG GCAGCGTGGG ATTACCATTC 360
AAGCGGCAGT CACTTCCTTC CAGTGGCACA GATGTAAAGT TAACATTGTG GATACGCCCG 420
GCCACATGGA TTTTTTGGCG GAGGTGTACC GCTCTTTGGC TGTTTTAGAT GGGGCCATCT 480
TGGTGATCTC CGCTAAAGAT GGCGTGCAGG CCCAGACCCG TATTCTGTTC CATGCCCTGC 540
GGAAAATGAA CATTCCCACC GTTATCTTTA TCAACAAGAT CGACCAGGCT GGCGTTGATT 600
TGCAGAGCGT GGTTCAGTCT GTTCGGGATA AGCTCTCCGC CGATATTATC ATCAAGCAGA 660
CGGTGTCGCT GTCCCCGGAA ATAGTCCTGG AGGAAAATAC CGACATAGAA GCATGGGATG 720
CGGTCATCGA AAATAACGAT GAATTATTGG AAAAGTATAT CGCAGGAGAA CCAATCAGCC 780
GGGAAAAACT TGCGCGGGAG GAACAGCAGC GGGTTCAAGA CGCCTCCCTG TTCCCAGTCT 840
ATCATGGCAG CGCCAAAAAT GGCCTTGGCA TTCAACCGTT GATGGATGCG GTGACAGGGC 900
TGTTCCAACC GATTGGGGAA CAGGGGGGCG CCGCCCTATG CGGCAGCGTT TTCAAGGTTG 960
AGTACACCGA TTGCGGCCAG CGGCGTGTCT ATCTACGGTT ATACAGCGGA ACGCTGCGCC 1020
TGCGGGATAC GGTGGCCCTG GCCGGGAGAG AAAAGCTGAA AATCACAGAG ATGCGTATTC 1080
CATCCAAAGG GGAAATTGTT CGGACAGACA CCGCTTATCA GGGTGAAATT GTTATCCTTC 1140
CCAGCGACAG CGTGAGGTTA AACGATGTAT TAGGGGACCA AACCCGGCTC CCTCGTAAAA 1200
GGTGGCGCGA GGACCCCCTC CCCATGCTGC GGACGACGAT TGCGCCGAAA ACGGCAGCGC 1260
AAAGAGAACG GCTGCTGGAC GCTCTTACGC AACTTGCGGA TACTGACCCG CTTTTGCGTT 1320
GCGAAGTGGA TTCCATCACC CATGAGATCA TTCTTTCTTT TTTGGGCCGG GTGCAGTTGG 1380
AGGTTGTTTC CGCTTTGCTG TCGGAAAAAT ACAAGCTTGA AACAGTGGTA AAGGAACCCT 1440
CCGTCATTTA TATGGAGCGG CCGCTCAAAG CAGCCAGCCA CACCATCCAT ATCGAGGTGC 1500
CGCCCAACCC GTTTTGGGCA TCCATAGGAC TGTCTGTTAC ACCACTCTCG CTTGGCTCCG 1560
GTGTACAATA CGAGAGCCGG GTTTCGCTGG GATACTTGAA CCAGAGTTTT CAAAACGCTG 1620
TCAGGGATGG TATCCGTTAC GGGCTGGAGC AGGGCTTGTT CGGCTGGAAC GTAACGGACT 1680
GTAAGATTTG CTTTGAATAC GGGCTTTATT ACAGTCCGGT CAGCACGCCG GCGGACTTCC 1740
GCTCATTGGC CCCGATTGTA TTGGAACAGG CATTGAAGGA ATCGGGGACG CAGCTGCTGG 1800
AACCTTATCT CTCCTTCATC CTCTATGCGC CCCAGGAATA CCTTTCCAGG GCTTATCATG 1860
ATGCACCGAA ATACTGTGCC ACCATCGAAA CGGCCCAGGT AAAAAAGGAT GAAGTTGTCT 1920
TTACTGGCGA GATTCCCGCC CGCTGTATAC AGGCATACCG TACTGATCTG GCCTTTTACA 1980
CCAACGGGCG GAGCGTATGC CTTACAGAGC TGAAAGGATA TCAGGCCGCT GTCGGTCAGC 2040
CGGTCATCCA GCCCCGCCGT CCAAACAGCC GCCTGGACAA GGTGCGCCAT ATGTTTCAGA 2100
AGGTAATGTA AAGATACATA ATCGTCAAGA CGGCAACAAT CAGAAGTTAT GGAGCACCGA 2160
TGGTAATTCT AGTATGTTCC GATAAAAATG CTGCAAGTAA TTTTCAGGGT GGAAATAGCT 2220
ATTTGACAGA TGGTGTTATT GCAGCAACAC ATATGATGCT GGCAGCAACA GATATAGGAT 2280
TGGATACATG TTGGGCTGGA GTGAATGATG TTCTTAAAAC ACAGAATGTT TTTGATTTGC 2340
CAGAAAACAT CTATCCTATT TGTTTCCTGG ATTTGGGATT TCGCGCT 2387
//