Class Y (IncQ plasmid pIE1120 - tet(Y) gene)
ID AF070999 standard; DNA; UNC; 3306 BP.
XX
AC AF070999;
XX
NI g3309048
XX
DT 13-JUL-1998 (Rel. 56, Created)
DT 11-NOV-1998 (Rel. 57, Last updated, Version 2)
XX
DE IncQ plasmid pIE1120 mobilization protein MobA (mobA) gene, partial
DE cds; and mobilization protein MobC (mobC) and tetracycline
DE resistance protein (tetA, class Y) genes, complete cds.
XX
KW .
XX
OS IncQ plasmid pIE1120
OC broad host range plasmids.
XX
RN [1]
RP 1-3306
RA Tietze E.;
RT "Tet Y, a novel tetracycline resistance determinant encoded by the IncQ
RT plasmid pIE1120";
RL Unpublished.
XX
RN [2]
RP 1-3306
RA Tietze E.;
RT ;
RL Submitted (05-JUN-1998) to the EMBL/GenBank/DDBJ databases.
RL Wernigerode, Robert Koch-Institut, Burgstrasse, Wernigerode D 38843,
RL Germany
XX
FH Key Location/Qualifiers
FH
FT source 1..3306
FT /organism="IncQ plasmid pIE1120"
FT /note="broad-host-range plasmid"
FT /specific_host="Eschericia coli"
FT misc_feature complement(1..1462)
FT /note="similar to IncQ plasmid RSF1010 in GenBank Accession
FT Number M28829"
FT CDS complement(<1..122)
FT /codon_start=1
FT /db_xref="PID:g3309051"
FT /transl_table=11
FT /gene="mobA"
FT /product="mobilization protein MobA"
FT /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVL"
FT rep_origin complement(203..290)
FT /note="oriT; similar to the oriT of IncQ plasmid RSF1010 in
FT GenBank Accession Number M28829"
FT CDS 321..605
FT /codon_start=1
FT /db_xref="PID:g3309050"
FT /transl_table=11
FT /gene="mobC"
FT /product="mobilization protein MobC"
FT /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
FT VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
FT rep_origin complement(630..1025)
FT /note="oriV; similar to the oriV of IncQ plasmid RSF1010 in
FT GenBank Accession Number M28829"
FT CDS 1680..2855
FT /codon_start=1
FT /db_xref="PID:g3309049"
FT /note="similar to Vibrio anguillarum TetA class G protein
FT in GenBank Accession Number S52437"
FT /transl_table=11
FT /gene="tetA(Y)"
FT /function="tetracycline/metal-proton antiporter"
FT /product="tetracycline resistance protein"
FT /translation="MSKSLITALIVVALDAIGLGLIMPVVPALLNEFVPAEQTAFHYGV
FT FLSLYAFMQVFCAPVLGRLSDRYGRRIILLVSFLGATIDYSIMAAAPVLWVLYIGRIIS
FT GVTGATGAIAASIIADTTKQEERARWFGFMGACFGAGMIAGPAIGGVLGDISVHAPFVA
FT GALLNAIAFCLVAFLLPKTPSQPPEGQPAKINLFEGFRFNFAVQGLASFFALFFLMQLI
FT GQAPAALWVIYGEQRLNWDIGTAGVSLAVFGAAHTFVQAVLTGTLSKRLGDRGVLLLGM
FT GADMCGFLLLAFITQSWMVLPAIFMLATGGIGMPALQAIISGLVCDEKQGALQGTLTGL
FT TNITSIIGPVGFTTLYGLTAGQWDGWVWLVAASLYLIAIPLLRQSASLLRS"
XX
SQ Sequence 3306 BP; 682 A; 782 C; 958 G; 884 T; 0 other;
TGCAAGACTT CATCCATGTC GCGGGCATAC TTGCCTTCGC GCTGGATGTA GTCGGCCTTG 60
GCCCTGGCCG ATTGGCCGCC CGACCTGCTG CCGGTTTTCG CCGTAAGGTG ATAAATCGCC 120
ATGCTGCCTC GCTGTTGCTT TTGCTTTTCG GCTCCATGCA ATGGCCCTCG GAGAGCGCAC 180
CGCCCGAAGG GTGGCCGTTA GGCCAGTTTC TCGAAGAGAA ACCGGTAAGT GCGCCCTCCC 240
CTACAAAGTA GGGTCGGGAT TGCCGCCGCT GTGCCTCCAT GATAGCCTAC GAGACAGCAC 300
ATTAACAATG GGGTGTCAAG ATGGTTAAGG GGAGCAACAA GGCGGCGGAT CGGCTGGCCA 360
AGCTCGAAGA ACAACGAGCG CGAATCAATG CCGAAATTCA GCGGGTGCGG GCAAGGGAAC 420
AGCAGCAAGA GCGCAAGAAC GAAACAAGGC GCAAGGTGCT GGTGGGGGCC ATGATTTTGG 480
CCAAGGTGAA CAGCAGCGAG TGGCCGGAGG ATCGGCTCAT GGCGGCAATG GATGCGTACC 540
TTGAACGCGA CCACGACCGC GCCTTGTTCG GTCTGCCGCC ACGCCAGAAG GATGAGCCGG 600
GCTGAATGAT CGACCGAGAC AGGCCCTGCG GGGCTGCACA CGCGCCCCCA CCCTTCGGGT 660
AGGGGGAAAG GCCGCTAAAG CGGCTAAAAG CGCTCCAGCG TATTTCTGCG GGGTTTGGTG 720
TGGGGTTTAG CGGGCTTTGC CCGCCTTTCC CCCTGCCGCG CAGCGGTGGG GCGGTGTGTA 780
GCCTAGCGCA GCGAATAGAC CAGCTATCCG GCCTCTGGCC GGGCATATTG GGCAAGGGCA 840
GCAGCGCCCC ACAAGGGCGC TGATAACCGC GCCTAGTGGA TTATTCTTAG ATAATCATGG 900
ATGGATTTTT CCAACACCCC GCCAGCCCCC GCCCCTGCTG GGTTTGCAGG TTTGGGGGCG 960
TGACAGTTAT TGCAGGGGTT CGTGACAGTT ATTGCAGGGG GGCGTGACAG TTATTGCAGG 1020
GGTTCGTGAC AGTTAGTACG GGAGTGACGG GCACTGGCTG GCAATGTCTA GCAACGGCAG 1080
GCATTTCGGC TGAGGGTAAA AGAACTTTCC GCTAAGCGAT AGACTGTATG TAAACACAGT 1140
ATTGCAAGGA CGCGGAACAT GCCTCATGTG GCGGCCAGGA CGGCCAGCCG GGATCGGGAT 1200
ACTGGTCGTT ACCAGAGCCA CCGACCCGAG CAAACCCTTC TCTATCAGAT CGTTGACGAG 1260
TATTACCCGG CATTCGCTGC GCTTATGGCA GAGCAGGGAA AGGAATTGCC GGGCTATGTG 1320
CAACGGGAAT TTGAAGAATT TCTCCAATGC GGGCGGCTGG AGCATGGCTT TCTACGGGTT 1380
CGCTGCGAGT CTTGCCACGC CGAGCACCTG GTCGCTTTCA GCTGTAAGCG TCGCGGTTTC 1440
TGCCCGAGCT GTGGGGCGCG GCTTCTTTAA ACTCAGCTGT ATAGGCTTTC TTCTGACTCA 1500
TGACACACAC CTTCTCTGGG CAGGTGATCT TACCTGCGTC TGTGTGTCTG GGGAACTATA 1560
GCCACTTCAT AACGGTCTCA CGATCCAATT TGGTCATTTT ATTGCATTAC CTCTTGACAA 1620
TTTATCGTTG ATAAAGTTTT TTATCGCCAA CTTATCAATG ATAAATAAAA GTCACATCTA 1680
TGTCAAAATC ACTTATAACC GCACTCATTG TTGTCGCGCT TGATGCGATT GGTTTGGGAT 1740
TAATCATGCC GGTGGTTCCG GCTTTATTAA ATGAATTTGT ACCGGCAGAG CAAACAGCAT 1800
TTCACTATGG TGTTTTTTTA TCGCTTTATG CGTTTATGCA GGTCTTTTGC GCGCCCGTTT 1860
TAGGGCGGTT ATCTGACCGC TATGGACGGC GGATTATTTT GCTGGTTTCA TTTTTAGGTG 1920
CCACGATTGA TTATAGCATA ATGGCGGCAG CGCCTGTTTT ATGGGTGCTT TATATCGGCC 1980
GGATTATCTC AGGTGTTACC GGAGCAACTG GTGCAATCGC GGCATCAATT ATCGCTGATA 2040
CAACTAAACA GGAAGAACGT GCGCGTTGGT TTGGTTTTAT GGGGGCGTGT TTTGGTGCAG 2100
GTATGATTGC AGGGCCTGCT ATTGGCGGTG TTCTTGGTGA TATATCTGTG CATGCGCCCT 2160
TTGTGGCAGG GGCTCTTCTC AATGCAATTG CCTTTTGTTT GGTGGCTTTC TTGTTGCCCA 2220
AAACGCCGTC ACAACCGCCT GAAGGACAGC CAGCCAAAAT CAATTTGTTT GAAGGCTTTC 2280
GCTTCAATTT TGCAGTTCAG GGACTTGCCA GCTTTTTTGC GTTGTTTTTT CTTATGCAGC 2340
TGATTGGGCA GGCGCCCGCC GCTTTGTGGG TGATTTATGG CGAACAGCGC TTGAATTGGG 2400
ATATTGGCAC AGCAGGTGTG TCGCTGGCCG TTTTTGGTGC AGCACATACA TTCGTACAAG 2460
CTGTTTTAAC CGGCACTCTT TCAAAGCGAC TGGGTGACCG CGGTGTGTTG CTGCTTGGAA 2520
TGGGCGCTGA TATGTGCGGG TTTTTATTGC TGGCTTTTAT CACGCAAAGC TGGATGGTTC 2580
TGCCGGCAAT TTTTATGCTG GCCACAGGCG GCATTGGTAT GCCTGCTTTG CAGGCCATTA 2640
TTTCAGGTCT TGTTTGCGAT GAAAAACAAG GTGCTTTACA GGGAACTTTA ACGGGGTTGA 2700
CGAATATAAC CTCGATTATC GGGCCGGTCG GATTTACGAC GCTTTATGGC TTAACCGCGG 2760
GGCAGTGGGA TGGTTGGGTT TGGCTCGTCG CAGCAAGCCT TTATCTTATT GCTATACCAT 2820
TATTGCGCCA GTCAGCCAGT TTATTGCGAT CTTAAAATCT TGGCGGGAGA TCAAGCAATC 2880
CCGCCAATAA AACCGCTGAG TTCATCAAGA CGCACCATTG CCATGCGCAT GGTGCCACGG 2940
CAATTTTGAG CTTTGCAGCG TGCGCGTCGG TTCAACTTCG TCCAAATATA ATTGCTCGTG 3000
TCCTCGAATT GAGCTGATCA TTGCCTTATT GATATAAACA AGGCGGTTAC ATTTCTTGCA 3060
GGTCATTTCT AATCTTTGAT GGTCTGCAAG ATCACTGGCT TTATAGCGGG TTTTCCAGTT 3120
TCCGGGTGGG GATGCCATTT TCATCACCAG AAGTCTTCAG GTGAGGGAAT ACGGACATAG 3180
CTGATTTTAC CGCCCACATG GGGTCGTTTG CGGGAGAGGG CGAAATCCTA CGCTAAGGCT 3240
TTGGCCAACG ATATTCTCCG GTAAGATTGA TGTGTTCCCA GGGGATAGGA GAAGTCGCTT 3300
GATATC 3306
//