otrA gene - Streptomyces rimosus
ID SROTRA standard; DNA; PRO; 2657 BP.
XX
AC X53401;
XX
NI g47472
XX
DT 06-FEB-1992 (Rel. 30, Created)
DT 06-FEB-1992 (Rel. 30, Last updated, Version 1)
XX
DE S.rimosus otrA gene for oxytetracycline resistance
XX
KW otr gene; oxytetracycline resistance.
XX
OS Streptomyces rimosus
OC Eubacteria; Firmicutes; Actinomycetes; Streptomycetes;
OC Streptomycetaceae; Streptomyces.
XX
RN [1]
RP 1-2657
RA Hunter I.S.;
RT ;
RL Submitted (09-JUN-1990) to the EMBL/GenBank/DDBJ databases.
RL Hunter I.S., University of Glasgow, Department of Genetics, Church
RL Street, Glasgow, g11 5js, UK.
XX
RN [2]
RP 1-2657
RX MEDLINE; 92236410.
RA Doyle D., McDowall K.J., Butler M.J., Hunter I.S.;
RT "Characterization of an oxytetracycline resistance gene, otrA, of
RT Streptomyces rimosus";
RL Mol. Microbiol. 5:2923-2933(1991).
XX
DR SPTREMBL; Q55002; Q55002.
XX
CC Sequence identity with tetM & tetO
XX
FH Key Location/Qualifiers
FH
FT source 1..2657
FT /organism="Streptomyces rimosus"
FT /isolate="15883R"
FT -35_signal 186..191
FT TATA_signal 212..216
FT prim_transcript 220..2393
FT /gene="otrA"
FT CDS 349..2340
FT /gene="otrA"
FT /function="oxytetracycline resistance"
FT /db_xref="PID:g47473"
FT /db_xref="SPTREMBL:Q55002"
FT /translation="MNKLNLGILAHVDAGKTSLTERLLHRTGVIDEVGSVDAGTTTTDS
FT MELERQRGITIRSAVATFVLDDLKVNLIDTPGHSDFISEVERALGVLDGAVLVVSAVEG
FT VQPQTRILMRTLRRLGIPTLVFVNKIDRGGARPDGVLREIRDRLTPAAVALSAVADAGT
FT PRARAIALGPDTDPDFAVRVGELLADHDDAFLTAYLDEEHVLTEKEYAEELAAQTARGL
FT VHPVYFGSALTGEGLDHLVHGIRELLPSVHASQDAPLRATVFKVDRGARGEAVAYLRLV
FT SGTLGTRDSVTLHRVDHTGRVTEHAGRITALRVFEHGSATSETRATAGDIAQAWGLKDV
FT RVGDRAGHLDGPPPRNFFAPPSLETVIRPERPEEAGRLHAALRMLDEQDPSIDLRQDEE
FT NAAGAVVRLYGEVQKEILGSTLAESFGVRVRFDPTRTVCIEKPVGTGEALIELDTRTHN
FT YFWGAPWVCASDRPSPARAITFRLAVELGSLPLAFHKAIEETVHTTLRHGLYGWQVTDC
FT AVTLTRTGVRSPVSAADDFRKANARLVLMDALGRAGTEVHEPVSSFELEVPAARLSPVL
FT AKLAELGATPGVPTAEGDVFRLEGTMPTSLVHDFNQRVPGLTQGEGVFLAEHRGYRPAV
FT GQPPVRPRPEGPNPLNRDEYILHVLKRV"
FT terminator 2346..2393
XX
SQ Sequence 2657 BP; 406 A; 938 C; 933 G; 380 T; 0 other;
GGATCCGGCC ACCACGACGG TGCACGACGT ACTGCAACGG CTCAACCACG GCCTCATCCG 60
CGGCGACAGC TCGTCCAGCA GCGTCGAGGA GTACCGCGCG GAGATCGAGG AGGCCGGCTT 120
CCGGGTGCGG CAGGCGGAGC GCATCGACAA CCTCCTCGGT GACTGGCTGA TAGTCGCCGT 180
CAAGCCCTGA CCCTGCGTGG CCGCCCTTGC TACCGTGATC ATGCCCGCGG GTGTGTTCCC 240
GCCGGGCGGA TCACAGCGAC GTCAGAGAGG GCGGAACGAT GCGGCAGGCG ACCACGAATC 300
CGGCAGGAAA GGTCCGCATC ACGACGCTAA GGAATCCGAA CCCCCTCCAT GAACAAGCTG 360
AATCTGGGCA TCCTGGCCCA CGTTGACGCC GGCAAGACCA GCCTCACCGA GCGCCTGCTG 420
CACCGCACCG GTGTGATCGA CGAGGTCGGC AGCGTGGACG CCGGCACCAC GACGACCGAC 480
TCGATGGAGC TGGAGCGGCA GCGCGGCATC ACCATCCGGT CCGCCGTGGC CACGTTCGTC 540
CTGGACGATC TCAAGGTCAA CCTCATCGAC ACCCCGGGCC ACTCCGACTT CATCTCCGAG 600
GTCGAGCGGG CGCTCGGGGT GCTCGACGGC GCGGTCCTGG TGGTCTCGGC CGTCGAGGGC 660
GTCCAGCCGC AGACCCGCAT CCTGATGCGG ACCCTGCGCA GGCTGGGCAT TCCCACGCTG 720
GTCTTCGTCA ACAAGATCGA CCGGGGCGGC GCGCGTCCCG ACGGTGTGCT GCGGGAGATC 780
CGCGACCGGC TCACCCCCGC CGCGGTGGCA CTGTCCGCCG TGGCGGACGC CGGCACGCCG 840
CGGGCCCGCG CGATCGCGCT CGGCCCGGAC ACCGACCCGG ACTTCGCCGT CCGGGTCGGT 900
GAGCTGCTGG CCGACCACGA CGACGCGTTC CTCACCGCCT ACCTGGACGA GGAACACGTA 960
CTGACCGAGA AGGAGTACGC GGAGGAACTG GCCGCGCAGA CCGCGCGCGG TCTGGTGCAC 1020
CCGGTGTACT TCGGGTCCGC GCTGACCGGC GAGGGCCTGG ACCATCTGGT GCACGGCATC 1080
CGGGAGTTGC TGCCGTCCGT GCACGCGTCG CAGGACGCGC CGCTGCGGGC CACCGTGTTC 1140
AAGGTGGACC GTGGCGCGCG CGGCGAGGCC GTCGCGTACC TGCGGCTGGT CTCCGGCACG 1200
CTGGGCACCC GCGATTCGGT GACGCTGCAC CGCGTCGACC ACACCGGCCG GGTCACCGAG 1260
CACGCCGGAC GCATCACCGC GCTGCGGGTC TTCGAGCACG GGTCGGCCAC CAGCGAGACC 1320
CGGGCGACCG CCGGGGACAT CGCGCAGGCG TGGGGCCTGA AGGACGTACG GGTCGGTGAC 1380
CGGGCCGGGC ACCTCGACGG TCCCCCGCCG CGCAACTTCT TCGCGCCGCC CAGCCTGGAG 1440
ACCGTGATCA GGCCGGAGCG CCCGGAGGAA GCGGGACGGC TGCACGCCGC GCTGCGCATG 1500
CTGGACGAGC AGGACCCCTC GATCGACCTG CGGCAGGACG AGGAGAACGC GGCCGGCGCG 1560
GTGGTCCGCC TCTACGGGGA GGTGCAGAAG GAGATCCTCG GCAGCACGCT CGCGGAGTCC 1620
TTCGGCGTAC GGGTGCGCTT CGACCCGACC CGTACGGTCT GCATCGAAAA GCCCGTGGGG 1680
ACCGGCGAGG CGCTGATCGA GCTGGACACG CGGACGCACA ACTACTTCTG GGGCGCACCG 1740
TGGGTCTGCG CGTCGGACCG GCCGAGCCCG GCGCGGGCGA TCACGTTCCG TTTGGCGGTG 1800
GAACTGGGCT CGCTCCCCCT GGCCTTCCAC AAGGCCATCG AGGAGACGGT GCACACCACC 1860
CTGCGGCACG GTCTGTACGG CTGGCAGGTC ACCGACTGCG CCGTCACCCT GACCCGTACC 1920
GGCGTTCGCA GTCCGGTCAG CGCGGCCGAC GACTTCCGCA AGGCCAACGC GCGCTTGGTC 1980
CTGATGGACG CGCTCGGCAG GGCCGGTACG GAGGTGCACG AGCCGGTCAG CTCCTTCGAA 2040
CTGGAGGTGC CCGCCGCCCG GCTCAGCCCG GTACTTGCGA AACTCGCGGA ACTGGGCGCG 2100
ACGCCCGGTG TGCCCACGGC CGAGGGGGAC GTCTTCCGCC TGGAGGGCAC GATGCCGACC 2160
AGCCTCGTGC ACGACTTCAA CCAGCGGGTT CCCGGACTGA CCCAGGGCGA GGGCGTGTTC 2220
CTGGCCGAGC ACCGGGGCTA CCGGCCCGCC GTCGGACAGC CGCCCGTGCG GCCGCGGCCC 2280
GAGGGGCCCA ACCCGCTCAA CCGCGACGAG TACATCCTGC ACGTGCTCAA GCGCGTGTGA 2340
ACCGGCACCG GACCGCGCCC CGCGCGGATT TCCGCCCGGG GCGCGGTCCG GTGGTTTCAG 2400
GTGCCGGCCT GTACCTGGAG GCGGACCTCA GGCCGTGGAT ACGGGCTGTC CTGCCGTGCG 2460
TACGGGCCGG TGTTCCGGGA GATTCCGAGG ATCAGCATGG ATTTCGCCTT GTTCACGGCA 2520
TCGATCCGGG AAACCGCGCC CAGCTTTTCG AAAACATTGC TCAAATGGCG TTTCACGGTC 2580
GCTTCGGTGA TGGTGAGCCG GGCGGCGATC TGGCTGTTGC TCAGCCCGCG CGCCACGTTC 2640
TCCATTATCT GGAGCTC 2657
//