otrB gene - Streptomyces rimosus
LOCUS AF079900 1947 bp DNA BCT 18-SEP-1998
DEFINITION Streptomyces rimosus tetracycline efflux protein (otrB) gene,
complete cds.
ACCESSION AF079900
NID g3617953
KEYWORDS .
SOURCE Streptomyces rimosus.
ORGANISM Streptomyces rimosus
Eubacteria; Firmicutes; Actinobacteria; Streptomycetes;
Streptomycetaceae; Streptomyces.
REFERENCE 1 (bases 1 to 1947)
AUTHORS McMurry,L.M. and Levy,S.B.
TITLE Revised sequence of OtrB (Tet347) tetracycline efflux protein from
Streptomyces rimosus
JOURNAL Antimicrob. Agents Chemother. 42 (11) (1998) In press
REFERENCE 2 (bases 1 to 1947)
AUTHORS McMurry,L.M. and Levy,S.B.
TITLE Direct Submission
JOURNAL Submitted (23-JUL-1998) Dept of Molecular Biology/Microbiology,
Tufts University School of Medicine, 136 Harrison Av, Boston, MA
02111, USA
FEATURES Location/Qualifiers
source 1. .1947
/organism="Streptomyces rimosus"
/strain="PG3"
/db_xref="taxon:1927"
gene 40. .1731
/note="tet347"
/gene="otrB"
CDS 40. .1731
/gene="otrB"
/note="OtrB; membrane protein; contains 14 putative
transmembrane helices that cause 'self-resistance' to
tetracyclines in the antibiotic-producing host; uses GTG
start codon; see also GenBank Accession Numbers M20370
and AF061335"
/codon_start=1
/transl_table=11
/product="tetracycline efflux protein"
/db_xref="PID:g3617954"
/translation="MSSANPGPAGTADQAGGAFTHRQILTAMSGLLLAVFLAALDQTV
IATAMRTIADDLHGQTEQAWATTGYLIASVLAMPFYGKLSDIYGRKPMYLISIVVFIG
GSVLCGTAGSMWELALFRAVQGLGGGGLMSLPTAVVADLAPVRERGRYFAFLQMAWVV
ASVAGPLAGGFFAEAGQVFGIDGWRWVFLLNVPLGLLALVTVRKALNLPHERREHRMD
VLGAAALALFLVPLLIVAEQGRTWGWGSPAALALFALGAAGLAVFIPVELRRGDEAIL
PLGLFRRGSIALCSAVNFTIGVGIFGTVTTLPLFLQMVQGRTPTQAGLVVIPFMLGTI
ASQMVSGKLIASSGRFKKLAIVGLGSMAGALLAMATTGATTPMWGIVLIVLWLGVGIG
LSQTVITSPMQNSAPKSQLGVANGASGLCRQIGGSTGIAVLFSVMFAVALGRLADLLH
TPRYERLLTDPAITGDPANHRFLDMAESGQGAGINLDDTSLLNGIDARLMQPVTDSFA
HGFHIMFLAGGVVLLAGFVMTWFLRELQEETAPEEERPAESGAGAKNGPLPASDA"
BASE COUNT 236 a 715 c 671 g 325 t
ORIGIN
TGTACGGGCC GGAAGCGCCG GCCAGGAGAG TGAGGAACCG TGTCATCCGC AAATCCGGGC 60
CCGGCGGGCA CGGCGGACCA GGCAGGCGGG GCGTTCACGC ATCGGCAGAT CCTGACGGCC 120
ATGTCGGGAC TGCTGCTGGC CGTGTTCCTC GCGGCCCTGG ACCAGACGGT CATCGCCACC 180
GCGATGCGCA CCATCGCGGA CGACCTCCAC GGCCAGACCG AGCAGGCATG GGCGACGACG 240
GGCTACCTCA TCGCCTCCGT CCTGGCGATG CCGTTCTACG GCAAGCTGTC CGACATCTAC 300
GGGCGCAAGC CCATGTACCT GATCTCCATC GTGGTGTTCA TCGGCGGCTC GGTGCTGTGC 360
GGCACGGCCG GCTCGATGTG GGAGCTGGCC CTCTTCCGGG CCGTCCAGGG ACTGGGCGGC 420
GGCGGGCTGA TGTCCCTGCC CACCGCGGTG GTCGCCGACC TCGCCCCGGT GCGCGAGCGC 480
GGCCGCTACT TCGCCTTCCT CCAGATGGCG TGGGTGGTCG CCAGCGTCGC GGGCCCGCTG 540
GCGGGCGGCT TCTTCGCGGA GGCGGGCCAG GTCTTCGGCA TCGACGGCTG GCGCTGGGTG 600
TTCCTGCTCA ACGTACCGCT GGGCCTGCTG GCCCTGGTCA CCGTGCGCAA GGCCCTGAAC 660
CTGCCGCACG AACGGCGCGA GCACCGCATG GACGTACTGG GCGCGGCGGC GCTGGCGCTG 720
TTCCTGGTGC CCCTGCTGAT CGTCGCCGAA CAGGGCCGGA CCTGGGGCTG GGGCTCGCCG 780
GCCGCCCTCG CCCTCTTCGC GCTCGGCGCG GCCGGGCTGG CGGTCTTCAT CCCCGTCGAG 840
CTGCGGCGCG GCGACGAGGC CATCCTGCCG CTGGGGCTCT TCCGGCGCGG CAGCATCGCG 900
CTGTGCTCCG CGGTCAACTT CACCATCGGC GTCGGCATCT TCGGCACGGT CACCACCCTG 960
CCGCTGTTCC TCCAGATGGT GCAGGGGCGG ACCCCGACCC AGGCCGGACT GGTGGTCATC 1020
CCGTTCATGC TGGGCACCAT CGCCTCGCAG ATGGTCTCCG GCAAGCTCAT CGCGTCCTCG 1080
GGCCGGTTCA AGAAACTGGC GATCGTGGGC CTGGGCTCGA TGGCCGGGGC GCTGCTGGCC 1140
ATGGCCACCA CCGGCGCGAC GACCCCGATG TGGGGCATCG TCCTGATCGT CCTCTGGCTC 1200
GGCGTCGGCA TCGGCCTGTC CCAGACCGTC ATCACCTCGC CCATGCAGAA CTCGGCCCCC 1260
AAGAGCCAGC TCGGCGTGGC GAACGGCGCC TCCGGCCTGT GCCGGCAGAT CGGCGGCTCC 1320
ACCGGCATCG CGGTTCTGTT CTCCGTGATG TTCGCGGTGG CGCTCGGCCG CCTCGCCGAC 1380
CTGCTGCACA CCCCGCGCTA CGAGCGCCTC CTGACGGACC CGGCGATCAC CGGCGACCCC 1440
GCCAACCACC GCTTCCTTGA CATGGCCGAG TCCGGGCAGG GCGCGGGGAT CAACCTTGAC 1500
GACACGTCCC TGCTGAACGG CATCGACGCC CGGCTGATGC AGCCGGTGAC GGATTCCTTC 1560
GCCCACGGCT TCCACATCAT GTTCCTCGCC GGCGGCGTGG TGCTGCTGGC CGGGTTCGTC 1620
ATGACCTGGT TCCTGCGCGA ACTCCAGGAG GAGACCGCGC CGGAGGAGGA GCGGCCGGCC 1680
GAGAGCGGCG CCGGGGCGAA GAACGGGCCG CTGCCCGCGT CGGACGCCTG ACCGTACGAA 1740
GACGGGCTCC GGCACCCGTA CGACGCGCTC CGGCGCGGGG AACGTCCGAT TCCCCGTGCC 1800
GGAACGGCCG GTTCCCGGCT CCCGGCCGCC CGTGCCGGGG TGTTCTGCCA CCAAGGTTGC 1860
AGTTTCCCCT TCCGGTTTCC GCTGCCGGGC TGCACTTCCG TTGCCTTTGC TTGGGCCCCC 1920
GTTCCGCCGC CATTAGCGTC TGGATCC 1947
//