Tetracycline determinants: Class A

Tetracycline resistance gene classification: Class A

© Service of Biosafety & Biotechnology - Author: Jean-Marc Collard
Last revised: November 13, 1998

MECHANISM	Efflux	12-TMS (Transmembrane segments)
	Ribosomal protection
	Enzymatic
	Unknown

MINOCYCLINE RESISTANCE

YES

DISTRIBUTION

Gram-negatives: Aeromonas, Citrobacter, Edwardsiella, Escherichia, Klebsiella, Plesiomonas, Proteus, Pseudomonas, Salmonella, Serratia, Shigella, Vibrio

REPRESENTATIVES	RP1	Tn1721
GENETIC LOCATION	Plasmid	Transposon
SOURCE	E. coli	Gram-negative bacteria
SEQUENCE ACCESS NB	X00006 (Ref. 1)	X61367; S87924 (Ref. 1, 2)

PROTEIN WEIGHT	42.205 kDa (Calculated) (Ref. 1)
PROBES	750-bp SmaI fragment of pSL18 (Ref. 3, 4, 5) 300-bp NruI-SmaI fragment of pRU1000 (Ref. 6) GCCTCCTGCGCGATCTGG (1416-1433) (Ref. 7)
PCR PRIMERS	see reference 8

REFERENCES

1 Waters, S. H., P. Rogowsky, J. Grinsted, J. Altenbuchner, and R. Schmitt. 1983. The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis. Nucleic Acids Res. 11:6089-6105.

2 Allmeier, H., B. Cresnar, M. Greck, and R. Schmitt R. 1992. Complete nucleotide sequence of Tn1721: gene organization and a novel gene product with features of a chemotaxis protein. Gene 111:11-20.

3 Lee, C. Y., B. E. Langlois, and K. A. Dawson. 1993. Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure. Appl. Environ. Microbiol. 59:1467-1472.

4 Andersen, S. R, and R. A. Sandaa. 1994. Distribution of tetracycline resistance determinants among Gram-negative bacteria isolated from polluted and unpolluted marine-sediments. Appl. Environ. Microbiol. 60:908-912.

5 Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.

6 Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.

7 DePaola, A., W. E. Hill, and F. M. Harrel. 1993. Oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds. Mol. Cell. Probes 7:345-348.

8 Hansen, L. M., P. C. Blanchard, and D. C. Hirsh. 1996. Distribution of tet(H) among Pasteurella isolates from the United States and Canada. Antimicrob. Agents Chemother. 40:1558-1560.

	REFERENCES
1	Waters, S. H., P. Rogowsky, J. Grinsted, J. Altenbuchner, and R. Schmitt. 1983. The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis. Nucleic Acids Res. 11:6089-6105.
2	Allmeier, H., B. Cresnar, M. Greck, and R. Schmitt R. 1992. Complete nucleotide sequence of Tn1721: gene organization and a novel gene product with features of a chemotaxis protein. Gene 111:11-20.
3	Lee, C. Y., B. E. Langlois, and K. A. Dawson. 1993. Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure. Appl. Environ. Microbiol. 59:1467-1472.
4	Andersen, S. R, and R. A. Sandaa. 1994. Distribution of tetracycline resistance determinants among Gram-negative bacteria isolated from polluted and unpolluted marine-sediments. Appl. Environ. Microbiol. 60:908-912.
5	Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.
6	Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.
7	DePaola, A., W. E. Hill, and F. M. Harrel. 1993. Oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds. Mol. Cell. Probes 7:345-348.
8	Hansen, L. M., P. C. Blanchard, and D. C. Hirsh. 1996. Distribution of tet(H) among Pasteurella isolates from the United States and Canada. Antimicrob. Agents Chemother. 40:1558-1560.