Tetracycline resistance gene classification: Class B

© Service of Biosafety & Biotechnology - Author: Jean-Marc Collard
Last revised: November 13, 1998


MECHANISM

Efflux 12-TMS (Transmembrane segments)
    Ribosomal protection  
    Enzymatic  
    Unknown  

MINOCYCLINE RESISTANCE

YES

 

NO



DISTRIBUTION Gram-negatives: Actinobacillus, Aeromonas, Citrobacter, Enterobacter, Escherichia, Haemophilus, Klebsiella, Moraxella, Pasteurella, Plesiomonas, Proteus, Providencia, Salmonella, Serratia, Shigella, Treponema, Yersinia, Vibrio


REPRESENTATIVES Tn10
GENETIC LOCATION Transposon
SOURCE Escherichia coli
SEQUENCE ACCESS NB V00611 (Ref. 2)


PROTEIN WEIGHT 43.3 kDa (Calculated) (Ref. 2)
PROBES 1,275-bp HincII fragment of pRT29 (1, 3, 5)
1,275-bp HincII fragment of pRT11 (1, 5, 6)
CAGTGCTGTTGTTGTCATTAATA (275-297) (7)
PCR PRIMERS see reference 8


  REFERENCES
1 Jorgensen, R. A., and W. S. Reznikoff. 1979. Organisation of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10. J. Bacteriol. 138:705-714.
2 Hillen, W., and K. Schollmeier. 1983. Nucleotide sequence of the Tn10 encoded tetracycline resistance gene. Nucleic Acids Res. 11:525-539.
3 Lee, C. Y., B. E. Langlois, and K. A. Dawson. 1993. Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure. Appl. Environ. Microbiol. 59:1467-1472.
4 Andersen, S. R, and R. A. Sandaa. 1994. Distribution of tetracycline resistance determinants among Gram-negative bacteria isolated from polluted and unpolluted marine-sediments. Appl. Environ. Microbiol. 60:908-912.
5 Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.
6 Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.
7 DePaola, A., W. E. Hill, and F. M. Harrel. 1993. Oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds. Mol. Cell. Probes 7:345-348.
8 Hansen, L. M., P. C. Blanchard, and D. C. Hirsh. 1996. Distribution of tet(H) among Pasteurella isolates from the United States and Canada. Antimicrob. Agents Chemother. 40:1558-1560.