Tetracycline determinants: Class D

Tetracycline resistance gene classification: Class D

© Service of Biosafety & Biotechnology - Author: Jean-Marc Collard
Last revised: November 17, 1998

MECHANISM	Efflux	12-TMS (Transmembrane segments)
	Ribosomal protection
	Enzymatic
	Unknown

MINOCYCLINE RESISTANCE

YES

DISTRIBUTION

Gram-negatives: Aeromonas, Citrobacter, Edwardsiella, Enterobacter, Escherichia, Klebsiella, Pasteurella, Plesiomonas, Salmonella, Shigella, Vibrio, Yersinia¹¹

REPRESENTATIVES	RA1 (Ref. 1)	pIP173 (Ref. 2)
GENETIC LOCATION	Plasmid	Chromosome-Plasmid / IS 26
SOURCE	Aeromonas sp.	Salmonella ordonez BM200
SEQUENCE ACCESS NB	L06798 (Ref. 4)	X65876 (Ref. 5)

REPRESENTATIVES	pSP9351 (Ref. 3)
GENETIC LOCATION	Plasmid / IS26
SOURCE	Pasteurella piscida SP9351
SEQUENCE ACCESS NB	D16172 (Ref. 3)

PROTEIN WEIGHT	41 kDa (Calculated)
PROBES	3,050-bp HindIII-PstI fragment of pSL106 (6, 7, 8, 9)
PCR PRIMERS	see reference 10

REFERENCES

1 Marshall, B., C. Tachibana, and S. B. Levy. 1983. Frequency of tetracycline resistance determinant classes among lactose fermenting coliforms. Antimicrob. Agents Chemother. 24:835-840.

2 Labigne-Roussel, A. A., G. Gerbaud, and P. Courvalin. 1981. Translocation of sequences encoding antibiotic resistance from the chromosome to a receptor plasmid in Salmonella ordonez. Mol. Gen. Genet. 182:390-408.

3 Kim, E. H., and T. Aoki. 1994. The transposon-like structure of IS26-tetracycline, and kanamycin resistance determinant derived from transferable R plasmid of fish pathogen, Pasteurella piscicida. Microbiol. Immunol. 38:31-38.

4 Varela, M. F., and J. K. Griffith. 1993. Nucleotide and deduced protein sequences of the Class D tetracycline resistance determinant: Relationship to other antimicrobial transport proteins. Antimicrob. Agents Chemother. 37:1253-1258.

5 Allard, J. D., M. L. Gibson, L. H. Vu, T. T. Nguyen, and K. P. Bertrand. 1993. Nucleotide sequence of class D tetracycline resistance genes from Salmonella ordonez. Mol. Gen. Genet. 237:301-305.

6 Lee, C. Y., B. E. Langlois, and K. A. Dawson. 1993. Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure. Appl. Environ. Microbiol. 59:1467-1472.

7 Andersen, S. R, and R. A. Sandaa. 1994. Distribution of tetracycline resistance determinants among Gram-negative bacteria isolated from polluted and unpolluted marine-sediments. Appl. Environ. Microbiol. 60:908-912.

8 Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.

9 Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.

10 Hansen, L. M., P. C. Blanchard, and D. C. Hirsh. 1996. Distribution of tet(H) among Pasteurella isolates from the United States and Canada. Antimicrob. Agents Chemother. 40:1558-1560.

11 Galimand, M., A. Guiyoule, G. Gerbaud, B. Rasoamanana, S. Chanteau, E. Carniel, and P. Courvalin. 1997. Multidrug resistance in Yersinia pestis mediated by a transferable plasmid. N. Engl. J. Med. 337:702-704.

	REFERENCES
1	Marshall, B., C. Tachibana, and S. B. Levy. 1983. Frequency of tetracycline resistance determinant classes among lactose fermenting coliforms. Antimicrob. Agents Chemother. 24:835-840.
2	Labigne-Roussel, A. A., G. Gerbaud, and P. Courvalin. 1981. Translocation of sequences encoding antibiotic resistance from the chromosome to a receptor plasmid in Salmonella ordonez. Mol. Gen. Genet. 182:390-408.
3	Kim, E. H., and T. Aoki. 1994. The transposon-like structure of IS26-tetracycline, and kanamycin resistance determinant derived from transferable R plasmid of fish pathogen, Pasteurella piscicida. Microbiol. Immunol. 38:31-38.
4	Varela, M. F., and J. K. Griffith. 1993. Nucleotide and deduced protein sequences of the Class D tetracycline resistance determinant: Relationship to other antimicrobial transport proteins. Antimicrob. Agents Chemother. 37:1253-1258.
5	Allard, J. D., M. L. Gibson, L. H. Vu, T. T. Nguyen, and K. P. Bertrand. 1993. Nucleotide sequence of class D tetracycline resistance genes from Salmonella ordonez. Mol. Gen. Genet. 237:301-305.
6	Lee, C. Y., B. E. Langlois, and K. A. Dawson. 1993. Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure. Appl. Environ. Microbiol. 59:1467-1472.
7	Andersen, S. R, and R. A. Sandaa. 1994. Distribution of tetracycline resistance determinants among Gram-negative bacteria isolated from polluted and unpolluted marine-sediments. Appl. Environ. Microbiol. 60:908-912.
8	Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.
9	Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.
10	Hansen, L. M., P. C. Blanchard, and D. C. Hirsh. 1996. Distribution of tet(H) among Pasteurella isolates from the United States and Canada. Antimicrob. Agents Chemother. 40:1558-1560.
11	Galimand, M., A. Guiyoule, G. Gerbaud, B. Rasoamanana, S. Chanteau, E. Carniel, and P. Courvalin. 1997. Multidrug resistance in Yersinia pestis mediated by a transferable plasmid. N. Engl. J. Med. 337:702-704.