Tetracycline determinants: Class E

Tetracycline resistance gene classification: Class E

© Service of Biosafety & Biotechnology - Author: Jean-Marc Collard
Last revised: November 13, 1998

MECHANISM	Efflux	12-TMS (Transmembrane segments)
	Ribosomal protection
	Enzymatic
	Unknown

MINOCYCLINE RESISTANCE

YES

DISTRIBUTION

Gram-negatives: Aeromonas, marine Alcaligenes (Ref. 4), Escherichia, Providencia, Pseudomonas (Ref. 4), Serratia, Shigella, Vibrio

REPRESENTATIVES	pSL1456 (Ref. 1)
GENETIC LOCATION	Plasmid
SOURCE	Escherichia coli SLH1456A
SEQUENCE ACCESS NB	L06940 (Ref. 2)

PROTEIN WEIGHT	43.469 kDa (Calculated) (Ref. 2)
PROBES	2,500-bp ClaI-PvuI fragment of pSL1504 (3, 4, 5, 6)
PCR PRIMERS	see reference 7

	REFERENCES
1	Marshall, B., S. Morrissey, P. Flynn, and S. B. Levy. 1987. A new tetracycline-resistance determinant, class E, isolated from Enterobacteriaceae. Gene 50:11-117.
2	Allard, J. D., and K. P. Bertrand. 1993. Sequence of a class E tetracycline resistance gene from Escherichia coli and comparison of related tetracycline efflux proteins. J. Bacteriol. 175:4554-4560.
3	Lee, C. Y., B. E. Langlois, and K. A. Dawson. 1993. Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure. Appl. Environ. Microbiol. 59:1467-1472.
4	Andersen, S. R, and R. A. Sandaa. 1994. Distribution of tetracycline resistance determinants among Gram-negative bacteria isolated from polluted and unpolluted marine-sediments. Appl. Environ. Microbiol. 60:908-912.
5	Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.
6	Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.
7	Hansen, L. M., P. C. Blanchard, and D. C. Hirsh. 1996. Distribution of tet(H) among Pasteurella isolates from the United States and Canada. Antimicrob. Agents Chemother. 40:1558-1560.