Tetracycline determinants: Class K

Tetracycline resistance gene classification: Class K

© Service of Biosafety & Biotechnology - Author: Jean-Marc Collard
Last revised: November 13, 1998

MECHANISM	Efflux	14-TMS (Transmembrane segments)
	Ribosomal protection
	Enzymatic
	Unknown

MINOCYCLINE RESISTANCE

YES

DISTRIBUTION

Gram-positives: Bacillus, Clostridium, Enterococcus, Eubacterium, Haemophilus, Listeria, Mycobacterium, Nocardia, Peptostreptococcus, Staphylococcus, Streptococcus, Streptomyces

REPRESENTATIVES	pT181	pNS1
GENETIC LOCATION	Plasmid	Plasmid
SOURCE	Staphylococcus aureus	Staphylococcus aureus
SEQUENCE ACCESS NB	J01764; J01765 (Ref. 1, 2)	M16217 (Ref. 3)

PROTEIN WEIGHT	50.676 kDa (Calculated) (Ref. 2)
PROBES	870-bp HincII fragment of pT181 or pAT102 (Ref. 4, 5, 6) 760-bp ClaI-KpnI fragment from pST1 (Ref. 7)
PCR PRIMERS	see reference 5

REFERENCES

1 Khan, S. A., and R. P. Novick. 1983. Complete nucleotide sequence of pT181, a tetracycline resistance plasmid from Staphylococcus aureus. Plasmid 10:251-259.

2 Guay, G. G., S. A. Kahn, and D. M. Rothstein. 1993. The tet(K) gene of plasmid pT181 of Staphylococcus aureus encodes an efflux protein that contains 14 transmembrane helices. Plasmid 30:163-166.

3 Noguchi, N., T. Aoki, M. Sasatsu, M. Kono, K. Shishido, and T. Ando. 1986. Determination of the complete nucleotide sequence of pNS1, a staphylococcal tetracycline-resistance plasmid propagated in Bacillus subtilis. FEMS Microbiol. Lett. 37:283-288.

4 Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.

5 Pang, Y., B. A. Brown, V. A. Steingrube, R. J. Wallace, and M. C. Roberts. 1994. Tetracycline resistance determinants in Mycobacterium and Streptomyces species. Antimicrob. Agents Chemother. 38:1408-1412.

6 Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.

7 Schwarz. S., and W. C. Noble. 1994. Tetracycline resistance genes in staphylococci from the skin of pigs. J. Appl. Bacteriol. 76:320-326.

	REFERENCES
1	Khan, S. A., and R. P. Novick. 1983. Complete nucleotide sequence of pT181, a tetracycline resistance plasmid from Staphylococcus aureus. Plasmid 10:251-259.
2	Guay, G. G., S. A. Kahn, and D. M. Rothstein. 1993. The tet(K) gene of plasmid pT181 of Staphylococcus aureus encodes an efflux protein that contains 14 transmembrane helices. Plasmid 30:163-166.
3	Noguchi, N., T. Aoki, M. Sasatsu, M. Kono, K. Shishido, and T. Ando. 1986. Determination of the complete nucleotide sequence of pNS1, a staphylococcal tetracycline-resistance plasmid propagated in Bacillus subtilis. FEMS Microbiol. Lett. 37:283-288.
4	Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.
5	Pang, Y., B. A. Brown, V. A. Steingrube, R. J. Wallace, and M. C. Roberts. 1994. Tetracycline resistance determinants in Mycobacterium and Streptomyces species. Antimicrob. Agents Chemother. 38:1408-1412.
6	Roe, D. E., P. H. Braham, A. Weinberg, and M. C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.
7	Schwarz. S., and W. C. Noble. 1994. Tetracycline resistance genes in staphylococci from the skin of pigs. J. Appl. Bacteriol. 76:320-326.