Tetracycline resistance gene classification: Class O

© Service of Biosafety & Biotechnology - Author: Jean-Marc Collard
Last revised: March 3, 1998


MECHANISM Efflux
  Ribosomal protection
    Enzymatic
    Unknown

MINOCYCLINE RESISTANCE

YES

NO



DISTRIBUTION Gram-positives: Aerococcus, Enterococcus, Lactobacillus, Mobiluncus, Peptostreptococcus, Staphylococcus, Streptococcus
Gram-negatives: Campylobacter


REPRESENTATIVES pIP1433 pUA466
GENETIC LOCATION Plasmid Plasmid
SOURCE Campylobacter coli BM2509 Campylobacter jejuni
SEQUENCE ACCESS NB P23835 (Ref. 1) M18896 (Ref. 2)

REPRESENTATIVES tetO cloned in pDL421 tet(O)
GENETIC LOCATION Chromosome Chromosome
SOURCE Streptococcus mutans DL5 Streptococcus pneumoniae SA40300
SEQUENCE ACCESS NB M20925 (Ref. 3) Y07780 (Ref. 4)

PROTEIN WEIGHT 72.3 kDa (Calculated) (Ref. 2)
PROBES 1,458-bp HindIII-NdeI fragment of pIP143 (Ref.1, 4, 5, 6, 7)
1.8-kb HincII fragment of pUOA4 (Ref. 8)
PCR PRIMERS see reference 4

  REFERENCES
1 Sougakoff, W., B. Papadopoulou, P. Nordmann, and P. Courvalin. 1987. Nucleotide sequence and distribution of gene tetO encoding tetracycline resistance in Campylobacter coli. FEMS Microbiol. Lett. 44:153-159.
2 Manvathu, E.K., K. Hiratsuka, and D.E. Taylor. 1988. Nucleotide sequence analysis and expression of a tetracycline resistance gene from Campylobacter jejuni. Gene 62:17-26.
3 LeBlanc, D. J., L. N. Lee, B. M. Titmas, C. J. Smith, and F. C. Tenover. 1988. Nucleotide sequence analysis of tetracycline resistance gene tetO from Streptococcus mutans DL5. J. Bacteriol. 170:3618-3626.
4 Widdowson, C. A., K. P. Klugman, and D. Hanslo. 1996. Identification of the tetracycline resistance gene, tet(O) in Streptococcus pneumoniae. Antimicrob. Agents Chemother. 40:2891-2893.
5 Zilhao, R., B. Papadopoulou, and P. Courvalin. 1988. Occurence of the Campylobacter resistance gene tetO in Enterococcus and Streptococcus spp. Antimicrob. Agents Chemother. 32:1793-1796.
6 Charpentier, E., G. Gerbaud, and P. Courvalin. 1994. Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis. Antimicrob. Agents Chemother. 38:2330-2335.
7 Chaslus-Dancla, E., M.-C. Lesage-Descauses, S. Leroy-Sétrin, J.-L. Martel, and J.-P. Lafont. 1995. Tetracycline resistance determinants, Tet B and Tet M, detected in Pasteurella multocida from bovine herds. J. Antimicrob. Chemother. 36:815-819.
8 Roe, D.E., P.H. Braham, A. Weinberg, and M.C. Roberts. 1995. Characterization of tetracycline resistance in Actinobacillus actinomycetemcomitans. Oral Microbiol. Immunol. 10:227-232.