Tetracycline determinants: Class Q

Tetracycline resistance gene classification: Class Q

© Service of Biosafety & Biotechnology - Author: Jean-Marc Collard
Last revised: November 16, 1998

MECHANISM		Efflux
		Ribosomal protection
		Enzymatic
		Unknown

MINOCYCLINE RESISTANCE

YES

DISTRIBUTION

Gram-negatives: Bacteroides, Capnocytophaga, Mitsuokella, Porphyromonas, Prevotella, Veillonella
Gram-positives: Eubacterium, Lactobacillus, Mobiluncus, Peptostreptococcus, Streptococcus

REPRESENTATIVES	*tetQ*	tetA(Q)
GENETIC LOCATION	Conjugative transposon (Tc^R Em^R DOT) (Ref. 5)	Conjugative transposon
SOURCE	Bacteroides thetaiotaomicron DOT	Bacteroides fragilis 1126
SEQUENCE ACCESS NB	X58717 (Ref. 1)	Z21523 (Ref. 2)

REPRESENTATIVES	pRRI4 (Ref. 3)
GENETIC LOCATION	plasmid
SOURCE	Prevotella (formerly Bacteroides) ruminicola
SEQUENCE ACCESS NB	L33696 (Ref. 4)

PROTEIN WEIGHT	72.1 kDa (Calculated) (Ref. 1)
PROBES	0.9-kb EcoRI-EcoRV or 2.6-kb SstI fragment of pNFD13-2 (Ref. 1)
PCR PRIMERS	see reference 4

REFERENCES

1 Nikolich, M. P., N. B. Shoemaker, and A. A. Salyers. 1992. A Bacteroides tetracycline resistance gene represents a new class of ribosome protection tetracycline resistance. Antimicrob. Agents Chemother. 36:1005-1012.

2 Lépine, G., J.-M. Lacroix, C. B. Walker, and A. Progulske-Fox. 1993. Sequencing of a tet(Q) gene isolated from Bacteroides fragilis 1126. Antimicrob. Agents Chemother. 37:2037-2041.

3 Flint, H. J., A. M. Thompson, and J. Bisset. 1988. Plasmid-associated transfer of a tetracycline resistance in Bacteroides ruminicola. Appl. Environ. Microbiol. 54:855-860.

4 Nikolich, M. P., G. Hong, N. B. Shoemaker, and A. A. Salyers. 1994. Evidence for natural horizontal transfer of tetQ between bacteria that normally colonize humans and bacteria that normally colonize livestock. Appl. Environ. Microbiol. 60:3255-3260.

5 Li, L. Y., N. B. Shoemaker, and A.A. Salyers. 1995. Location and characteristics of the transfer region of a Bacteroides conjugative transposon and regulation of transfer genes. J. Bacteriol. 177:4992-4999.

6 Leng, Z., D. E. Riley, R. E. Berger, J. N. Krieger, and M. C. Roberts. 1997. Distribution and mobility of the tetracycline resistance determinant tetQ. J. Antimicrob. Chemother. 40:551-559.

	REFERENCES
1	Nikolich, M. P., N. B. Shoemaker, and A. A. Salyers. 1992. A Bacteroides tetracycline resistance gene represents a new class of ribosome protection tetracycline resistance. Antimicrob. Agents Chemother. 36:1005-1012.
2	Lépine, G., J.-M. Lacroix, C. B. Walker, and A. Progulske-Fox. 1993. Sequencing of a tet(Q) gene isolated from Bacteroides fragilis 1126. Antimicrob. Agents Chemother. 37:2037-2041.
3	Flint, H. J., A. M. Thompson, and J. Bisset. 1988. Plasmid-associated transfer of a tetracycline resistance in Bacteroides ruminicola. Appl. Environ. Microbiol. 54:855-860.
4	Nikolich, M. P., G. Hong, N. B. Shoemaker, and A. A. Salyers. 1994. Evidence for natural horizontal transfer of tetQ between bacteria that normally colonize humans and bacteria that normally colonize livestock. Appl. Environ. Microbiol. 60:3255-3260.
5	Li, L. Y., N. B. Shoemaker, and A.A. Salyers. 1995. Location and characteristics of the transfer region of a Bacteroides conjugative transposon and regulation of transfer genes. J. Bacteriol. 177:4992-4999.
6	Leng, Z., D. E. Riley, R. E. Berger, J. N. Krieger, and M. C. Roberts. 1997. Distribution and mobility of the tetracycline resistance determinant tetQ. J. Antimicrob. Chemother. 40:551-559.